FIGURE

Fig. 2

ID

ZDB-FIG-090519-8

Publication

Kitaguchi et al., 2009 - Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis

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Fig. 2

Identification of the cis-elements important for myeloid-specific lyz expression. (A) Nucleotide sequence of lyz promoter region between -1.9 kb and -1.7 kb. The red boxes and blue box indicate C/ebp family (CCAAT enhancer) and Runx family putative binding sequences, respectively. The arrows show the start positions of each deletion constructs. (B) Summary of EGFP transient expression assay. The embryos are classified as explained in Fig. 1. (C) Schematic map of the lyz promoter constructs lacking putative binding sites for Runx family, C/ebp family or Ets/Pu.1 family and summary of EGFP transient expression assay. (D) DNA pull-down assay of EGFP-fused transcriptional factors by the biotinated 2.4 kb lyz promoter fragments. EGFP-fusion constructs were expressed in HEK293T cells (bottom panel). The biotinated lyz promoter or mutant promoter was incubated with cell lysates and subsequently incubated with streptavidin beads. EGFP-runx1 precipitated with the lyz 2.4 kb promoter (F), but not the runx site deletion promoter (ΔR; top left panel). EGFP-c/ebp1 strongly precipitated with the lyz promoter (F), while EGFP-c/ebp1 weakly precipitated with the mutant promoter lacking c/ebp1 site at -1.46 kb (ΔC; top middle panel). EGFP-pu.1, but not EGFP-IRF8 precipitated with the lyz 2.4 kb promoter (top right panel).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 126(5-6), Kitaguchi, T., Kawakami, K., and Kawahara, A., Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis, 314-323, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.