FIGURE

Fig. 2

ID

ZDB-FIG-090508-11

Publication

Riley et al., 1997 - A critical period of ear development controlled by distinct populations of ciliated cells in the zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Distinct classes of cilia inside the otic vesicle. Embryos were fixed and incubated with primary antibodies directed against acetylated tubulin followed by fluorescent secondary antibodies. Stained embryos were then imaged by confocal microscopy to determine the distribution of cilia in the otic vesicle. (A) 19-h ear showing >100 cilia. (B) 24-h ear with two sets of presumed tethers (arrows) and numerous shorter cilia. (C) 27-h ear with two sets of tethers (arrows). Most shorter cilia have disappeared. (D) 30-h ear showing two sets of tethers. Anterior tethers are attached to hair cells the cell bodies of which stain intensely for acetylated tubulin. Posterior hair cells also stain positively but occur at a deeper (more medial) focal plane than is shown. Scale bar, 25 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 191(2), Riley, B.B., Zhu, C., Janetopoulos, C., and Aufderheide, K.J., A critical period of ear development controlled by distinct populations of ciliated cells in the zebrafish, 191-201, Copyright (1997) with permission from Elsevier. Full text @ Dev. Biol.