FIGURE

Fig. S5

ID

ZDB-FIG-090320-81

Publication

Jing et al., 2009 - Wnt signals organize synaptic prepattern and axon guidance through the zebrafish unplugged/MuSK receptor

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Fig. S5

Unplugged interacts with zebrafish Dishevelled.
(A) The intracellular domain of unplugged /MuSK (Unpic) was cloned into a bait plasmid pGBKT7. The PDZ and DEP domains of zebrafish dishevelled 2 and dishevelled 3 were cloned into a prey plasmid pGADT7. Two-hybrid interactions were preformed using SD/-Ade/-His/-Leu/-Trp media to screen for reporter genes: ADE2 and HIS3 (+++: fast growth. ++: intermediate growth. -: no growth). (B, C): The morphology and differentiation of adaxial cells expressing Myc-Xdsh-DEP+ appears normal. 28 hpf embryo injected with symhc1:myc-Xdsh-DEP+ and stained with mAb anti-Myc (red) and F59 (green, adaxial cells).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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