FIGURE

Fig. 6

ID

ZDB-FIG-090320-76

Publication

Jing et al., 2009 - Wnt signals organize synaptic prepattern and axon guidance through the zebrafish unplugged/MuSK receptor

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Fig. 6

Neuromuscular Synapses Form in the Absence of AChR Prepattern

Twenty-somite stage (A, B, G, and H) or 27 hpf (C–F, I, and J) embryos after heat-shock treatment.

(A and B) Tg(hsp70l:UnpSV1-myc;unplugged) embryos received heat shock from the 10 to 20 somite stage, which rescued AChR prepattern.

(C and D) Similar heat-shock treatment (10 somite to 27 hpf) also restored motor axon pathfinding, but not neuromuscular synapses.

(E and F) The same heat-shock treatment rescued motor axons and neuromuscular synapses in Tg(hsp70l:UnpFL-myc; unplugged) embryos.

(G and H) In contrast, heat shock between the 10 and 20 somite stage failed to rescue AChR prepattern in Tg(hsp70l:UnpFL-myc;unplugged) embryos.

(I and J) Heat-shock treatment of same embryos between the 26 somite stage and 27 hpf, i.e., after the time period of prepatterning, was sufficient to rescue neuromuscular synapses.

Scale bars, 50 μm.

Expression Data

Gene:	chrna1
Antibody:	znp-1
Fish:	musk^{tbr307/tbr307}; p145Tg musk^{tbr307/tbr307}; p146Tg
Condition:	heat shock
Anatomical Terms:	motor neuron axon myotome acetylcholine-gated channel complex
Stage Range:	20-25 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	musk^{tbr307/tbr307}; p145Tg musk^{tbr307/tbr307}; p146Tg
Condition:	heat shock
Observed In:	motor neuron axon guidance skeletal muscle acetylcholine-gated channel clustering
Stage Range:	20-25 somites to Prim-5

Phenotype Detail

Acknowledgments

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