Fig. 6

Inhibition of HSPC migration from the VDA to PBI blocks their differentiation into erythroid but not myeloid lineages. (A,B) Whole-mount in situ hybridization of runx1 expression in 30 hpf control MO injected vlt^m651 embryos (A) and sih MO injected vlt^m651 embryos (B). (C-H) Whole-mount in situ hybridization of cmyb expression in control MO-injected vlt^m651 embryos at 30 hpf (C), 3 dpf (E) and 4 dpf (G); sih MO injected vlt^m651 embryos at 30 hpf (D), 3 dpf (F) and 4 dpf (H). (I,J) Whole-mount in situ hybridization of βe1-globin expression in 4 dpf control MO injected vlt^m651 embryos (I) and sih MO-injected vlt^m651 embryos (J). (K,L) Whole-mount in situ hybridization of lyc expression in 4 dpf control MO-injected vlt^m651 embryos (K) and sih MO-injected vlt^m651 embryos (L). Insets are higher magnification (20x) views of the corresponding boxed regions (blue).