FIGURE

Fig. 4

ID

ZDB-FIG-081007-22

Publication

Mudumana et al., 2008 - odd skipped related1 reveals a novel role for endoderm in regulating kidney versus vascular cell fate

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Fig. 4

osr1 is required for glomerular morphogenesis. (A,B) Expression of wt1a in control (A) and osr1 morphants (B) at 26 hpf. (C,D) Histological cross-sections of the 26 hpf glomerular progenitors in wild-type (C) and osr1 morphants (D) show equivalent expression of wt1a (arrows). (E,F) Cross-section of wild-type glomerulus at 48 hpf (E) shows wt1a expression in the midline vascularized glomerulus (arrow), whereas in osr1 morphants (F) wt1a-positive podocytes remain in an immature state (arrows) and fail to coalesce to the midline. (G,H) Expression of the podocyte marker nephrin is lost in osr1 morphants (arrowhead, H) when compared with the control embryos (arrowhead, G). Segment-specific loss of ae2 expression in the proximal pronephros is evident in the osr1 morphants (H) when compared with controls (G) at 48 hpf.

Expression Data

Genes:	nphs1 slc4a2a wt1a
Fish:	AB/TU AB/TU + MO2-osr1
Knockdown Reagent:	MO2-osr1
Anatomical Terms:	pronephric distal early tubule pronephric distal late tubule pronephric glomerulus pronephric podocyte pronephric proximal convoluted tubule pronephric proximal straight tubule
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU + MO2-osr1
Knockdown Reagent:	MO2-osr1
Observed In:	pronephric glomerulus pronephric podocyte pronephric proximal convoluted tubule pronephros development
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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