FIGURE

Fig. 1

ID

ZDB-FIG-080923-26

Publication

Hashiguchi et al., 2008 - Nodal/Bozozok-independent induction of the dorsal organizer by zebrafish cell lines

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Fig. 1

Expression of specific marker genes in the secondary axis. (A, B) Dorsal telencephalic marker gene emx1. (C, D) Rhombomere 3 and 5 marker gene krx20. (E, F) Floor plate marker gene shh. (G, H) Somite marker gene myoD. (A, C, E, G) Control, non-implanted embryos. (B, D, F, H) cell-implanted embryos. The secondary axis had a visible notochord. (A–C, F–H) Lateral views. (D, E) Dorsal views. All embryos are at the pharyngula stage (24 hpf). Arrows indicate the ectopic expression of the marker gene inthe secondary axis. Scale bar, 100 μm.

Expression Data

Genes:	egr2b emx1 myod1 shha
Fish:	TU
Anatomical Terms:	dorsal telencephalon floor plate rhombomere 3 rhombomere 5 somite
Stage:	Prim-5

Expression Detail

Gene	Fish	Conditions	Stage	Anatomy	Assay
egr2b	TU	standard conditions	Prim-5	rhombomere 3	ISH
egr2b	TU	standard conditions	Prim-5	rhombomere 5	ISH
emx1	TU	standard conditions	Prim-5	dorsal telencephalon	ISH
myod1	TU	standard conditions	Prim-5	somite	ISH
shha	TU	standard conditions	Prim-5	floor plate	ISH

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Antibody Labeling

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Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 321(2), Hashiguchi, M., Shinya, M., Tokumoto, M., and Sakai, N., Nodal/Bozozok-independent induction of the dorsal organizer by zebrafish cell lines, 387-396, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.