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Fig. 1 Expression of specific marker genes in the secondary axis. (A, B) Dorsal telencephalic marker gene emx1. (C, D) Rhombomere 3 and 5 marker gene krx20. (E, F) Floor plate marker gene shh. (G, H) Somite marker gene myoD. (A, C, E, G) Control, non-implanted embryos. (B, D, F, H) cell-implanted embryos. The secondary axis had a visible notochord. (A–C, F–H) Lateral views. (D, E) Dorsal views. All embryos are at the pharyngula stage (24 hpf). Arrows indicate the ectopic expression of the marker gene inthe secondary axis. Scale bar, 100 μm.
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Reprinted from Developmental Biology, 321(2), Hashiguchi, M., Shinya, M., Tokumoto, M., and Sakai, N., Nodal/Bozozok-independent induction of the dorsal organizer by zebrafish cell lines, 387-396, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.