FIGURE

Fig. S1

ID

ZDB-FIG-080829-53

Publication

Kishi et al., 2008 - The identification of zebrafish mutants showing alterations in senescence-associated biomarkers

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Fig. S1

Pixel images for quantitation of SA-β-gal activity in zebrafish. (A–D) Colorimetric quantitation of SA-β-gal activity staining in the trunk sections of adult zebrafish. Lateral photographs were taken, and the area between the operculum and the dorsal and anal fins was chosen for quantitation (A and B). The blue pixel area was calculated (C and D as described in Materials and Methods), and SA-β-gal activity is expressed as a percentage of the total area values. The analysis was performed on both sides of each fish. Shown here are fish aged 5 months (A and C) and 57 months (B and D). E and F: Colorimetric quantitation of SA-β-gal activity in zebrafish embryos. The total blue pixel number was determined from lateral photographs of individual 3.5-day old zebrafish embryos. SA-β-gal staining intensities were quantified in untreated embryos (E) and embryos incubated in 500 mM BHP (F).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.