FIGURE

Fig. 3

ID

ZDB-FIG-080829-47

Publication

Kishi et al., 2008 - The identification of zebrafish mutants showing alterations in senescence-associated biomarkers

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Mutant nrs zebrafish show extremely high SA-β-gal activity and display both yolk and muscle phenotypes.
3.5-day old (3.5 dpf) homozygous nrs^{m/m (hi891/hi891)} zebrafish embryos show extremely high SA-β-gal activity (B) compared with wild-type embryos (A) (with PTU). The atp6v1h^{m/m (hi923/hi923)} mutant shown in (D) is another variant identified as having significantly higher SA-β-gal activity. Most other early embryonic lethal mutants derived from either insertional mutagenesis (as shown in Figure S4) and chemical mutagenesis (e.g., clo^m39/m39 which is shown in (C)) show no higher (or indistinguishable) SA-β-gal activity than wild-type siblings at any time during development. (E) Yolk opaque phenotypes can be observed in homozygous nrs^m/m embryos at 3.5 dpf (earliest detection at around 2.5 dpf; lower left panel), compared with wild-type embryos (upper left panel). Also shown is a comparison between the H&E staining of transverse sections of the yolk part of nrs^m/m embryos at 3.5 dpf (lower right panel) and wild-type embryos (upper right).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	atp6v1h^{hi923Tg/hi923Tg} spns1^{hi891Tg/hi891Tg} Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39
Observed In:	cellular senescence yolk
Stage Range:	Pec-fin to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.