FIGURE

Fig. 5

ID

ZDB-FIG-071127-22

Publication

diIorio et al., 2007 - TALE-Family homeodomain proteins regulate endodermal sonic hedgehog expression and pattern the anterior endoderm

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Fig. 5

Disruption of meis3 and pbx4 function causes an anterior shift of insulin-positive cells. Control (A), lzr mutant (B), δCPbx4-injected (C), MOCO-injected (D), tMO1-injected (E) and tMO2-injected (F) embryos, as well as embryos where MOCO (G) or tMO1 (H) was targeted to the endoderm, were assayed for insulin expression by in situ hybridization at 72 hpf. Note that all insulin positive cells are found anterior to somite 4 in experimental embryos (B, C, E, F, H), but not in control embryos (A, D, G). s1–s4 indicate the position of somites 1–4. All embryos are in lateral view with anterior to the left.

Expression Data

Gene:	ins
Fish:	WT pbx4^b557/b557 WT + MO1-meis3 WT + MO2-meis3
Condition:	chemical treatment: pharmaceutical
Knockdown Reagents:	MO1-meis3 MO2-meis3
Anatomical Term:	endocrine pancreas
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pbx4^b557/b557 WT + MO1-meis3 WT + MO2-meis3
Condition:	chemical treatment: pharmaceutical
Knockdown Reagents:	MO1-meis3 MO2-meis3
Observed In:	endocrine pancreas
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 304(1), diIorio, P., Alexa, K., Choe, S.K., Etheridge, L., and Sagerström, C.G., TALE-Family homeodomain proteins regulate endodermal sonic hedgehog expression and pattern the anterior endoderm, 221-231, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.