FIGURE

Fig. 2

ID

ZDB-FIG-070914-64

Publication

Steffen et al., 2007 - The zebrafish runzel muscular dystrophy is linked to the titin gene

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Fig. 2

Sarcomeric proteins are mislocalized in ruz mutant skeletal muscle. Indirect immunofluorescence was performed on longitudinal sections of 5–6 dpf mutant and wild-type skeletal muscle and samples were imaged at 100× (A, B) or 400x (C–F). (A, C) β-Dystroglycan protein localizes to the myosepta of wild-type embryonic muscle. (B, D) In ruz mutants, β-dystroglycan is maintained at the myosepta. (C) Nebulin localizes to the sarcomere in wild-type embryos. (D) Nebulin sarcomeric localization is lost throughout most of ruz mutant muscle. (E) Skeletal muscle actin similarly localizes to the sarcomere in wild-type embryos while localization is lost in ruz mutants (F). Nuclei were stained with DAPI (blue).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ruz^tk258/tk258 (TL)
Observed In:	skeletal muscle cell sarcomere
Stage Range:	Day 5 to Day 6

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 309(2), Steffen, L.S., Guyon, J.R., Vogel, E.D., Howell, M.H., Zhou, Y., Weber, G.J., Zon, L.I., and Kunkel, L.M., The zebrafish runzel muscular dystrophy is linked to the titin gene, 180-192, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.