Fig. 2

Detection of recombinant mouse Fibin expressed in COS-7 cells. (A) COS-7 cells were transfected with the empty vector (Control) or the mouse Fibin-expression vector (Fibin). The cell lysate (a, c) and culture medium (b, d) of the transfected COS-7 cells were separated by SDS–polyacrylamide gel electrophoresis under reducing conditions and transferred onto a nitrocellulose membrane. The protein with the E tag on the membrane was detected using anti-E tag antibody. (B) COS-7 cells transfected with the empty vector (Control) (a–f) or the mouse Fibin-expression vector (Fibin) (g–l) were examined by immunohistochemistry using anti-mannosidase II antibody for the Golgi apparatus (a, d, g, j) and anti-E tag antibody for recombinant Fibin (b, e, h, k). The signals obtained by immunohistochemistry using anti-mannosidase II antibody and anti-E tag antibody were merged (c, f, i, l). Scale bar = 20 μm.