Fig. 3

Expression of fibin in zebrafish embryos. (A) The spatiotemporal expression pattern of fibin in zebrafish embryos (10∼24 hpf) was examined by whole mount in situ hybridization. fibin was expressed in the notochord at 10 hpf (a) and in the notochord and somites at 12 and 14 hpf (b, c). fibin was also expressed in the lateral plate mesoderm, tail buds and the forebrain at 14 hpf (c, d). At 18 and 24 hpf, fibin was predominantly expressed in tail buds and presumptive pectoral fin bud regions. In addition, fibin was also expressed in the alar plate (e, f). (B) The expression of fibin in fibin MO-injected zebrafish embryos. The expression of fibin in wild-type (a), antisense five-base mismatched fibin (Control) MO- (b) and antisense fibin MO- (c) injected embryos at 18 hpf was examined by whole mount in situ hybridization. 1, notochord; 2, somites; 3, lateral plate mesoderm or presumptive pectoral fin bud regions; 4, tail; 5, forebrain; 6, alar plate of the hindbrain.