Fig. 5

Mutations affecting vascular patterning. (A–T) Bright-field and epifluorescent micrographs of Tg(flk1:EGFP)^s843unf^s808 mutant embryos (D, E, 30 hpf; F, 48 hpf), slv^s887 mutant (J–L, 78 hpf) and qad^s840 mutant (Q–R, 78 hpf) larvae and their wild-type (wt) siblings. (A–F) unf^s808 mutants have shortened intersegmental vessels (SEs, arrow in panel F) and fail to form the dorsal longitudinal anastomotic vessel (DLAV, arrowhead in panels C and F) and appear to have reduced sprouting angiogenesis in the head (E). (G–L) Mis-patterning of the intersegmental vessels (SEs, arrow in panel L) and failure to remodel the posterior cardinal vein (PCV, arrowhead in L) in slv^s887 mutant larvae. (M–S) Loop formation in the hindbrain capillary network in qad^s840 mutant larvae (P (wt) and T (qad^s840) are dorsal views focusing on the hindbrain capillary network). Instead of connecting lateral to medial as in wild-type (O, P), in qad^s840 mutant larvae capillaries connect back to their vessel of origin (arrowheads in panels S, T). Note that the tail vasculature in qad^s840 mutants (R) appears indistinguishable from wild-type (N). qad^s840 mutant embryos and their wild-type siblings were PTU treated to allow better visualization of the head vasculature.