FIGURE

Fig. 8

ID

ZDB-FIG-070608-13

Publication

Hall et al., 2007 - The zebrafish candyfloss mutant implicates extracellular matrix adhesion failure in laminin {alpha}2-deficient congenital muscular dystrophy

Other Figures

All Figure Page

Back to All Figure Page

Fig. 8

Expression of lama2 mRNA in the adaxial cells of a wild-type embryo and neuromuscular junction formation and primary motor neuron outgrowth. (A) In situ hybridization to Lama2 mRNA shows initial expression in the adaxial cells at the 10-somite stage. The adaxial cells are the first muscle cells of the embryo to differentiate. Wild-type, dorsal view, anterior to the left. (B) Wild type, lateral view, anterior to the left. (C) Staining with rhodamine-conjugated bungarotoxin allows visualization of the neuromuscular junctions (NMJs). No differences in the extent of NMJ formation were seen between caf embryos and wild-type siblings. Panels show confocal projections, lateral view, anterior to the left. Full projections are available as SI Movies 2 and 3. (D) Crossing caf zebrafish with the Tg(cmet:EGFP)ed6 line allowed visualization of the primary motor neurons. No defects in primary motor neuron formation or outgrowth were seen in caf embryos compared to wild-type siblings. Panels show confocal projections, lateral view, anterior to the left. Full projections are available as SI Movies 4 and 5.

Expression Data

Gene:	lama2
Fish:	WT
Anatomical Term:	adaxial cell
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lama2^{teg15a/teg15a} lama2^{teg15a/teg15a}; ed6Tg
Observed In:	primary motor neuron whole organism neuromuscular junction
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA