Fig. 2

Expression of anosmin-1a in sensory hair cells of the cristae and in the ampullary nerve of adult zebrafish inner ear. Whole-mount double immunofluorescence with anti-anosmin-1a polyclonal (green) and anti-acetylated tubulin monoclonal (blue) antibodies combined to rhodamin-phalloidin staining of actin (red). (A–D) In the whole cristae, anosmin-1a staining was observed at both extremities of the sensory patch and in the ampullary nerve. (E–H) At the extremity of the cristae, anosmin-1a is accumulated in the ciliated cells, mainly in the hair bundle but also in the cell body, and in the nerve fibers. (I–K) Higher magnification revealed anosmin-1a accumulation in the actin-rich hair bundle and also in the cell body. (L) Confocal section showing anosmin-1a staining of the cilia, in the supranuclear region and at the cell membrane. Data are maximum intensity projections of the acquired z-stacks (A–K) or a single confocal section (L). Scale bar: 50 μm (A–D), 10 μm (E–L).