FIGURE

Fig. 1

ID

ZDB-FIG-070118-4

Publication

Lin et al., 2006 - Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish

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Fig. 1

Tagging all head muscles by using the transgenic zebrafish line Tg(α-actin:RFP). Endogenous α-actin transcripts (A) and red fluorescent protein (RFP) reporter transcripts (B) were detected by whole-mount in situ hybridization of zebrafish embryos at 14 hpf (lateral view: A, B). RFP expressions in the embryos derived from the transgenic line (α-actin:RFP) at 22.5 hpf (C, D), 72 hpf (E, F), and 120 hpf (G, H) were observed from a lateral view (E, G) and from a ventral view (F, H). Panel D is a magnification of panel C. RFP appeared in the formed somites (D, arrowhead) but was absent in the newly forming somite (D, arrow) at 22.5 hpf. Meanwhile, all cranial muscles were labeled by red fluorescent signal in the embryos both at 72 and at 120 hpf. The muscles are designated following the scheme of Schilling and Kimmel (1997): ah, adductor hyoideus; am, adductor mandibulae; ao, adductor operculi; do, dilator operculi; dpw1–5, dorsal pharyngeal wall 1–5; hh, hyohyoideus; ih, interhyoideus; ima, intermandibularis anterior; imp, intermandibularis posterior; io, inferior oblique; ir, inferior rectus; lap, levator arcus palatini; lr, lateral rectus; mr, medial rectus; sh, sternohyoideus; so, superior oblique; sr, superior rectus; and tv 1–5, transvs. ventralis 1–5.

Expression Data

Gene:	actc1b
Fish:	WT
Anatomical Term:	somite
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 299(2), Lin, C.Y., Yung, R.F., Lee, H.C., Chen, W.T., Chen, Y.H., and Tsai, H.J., Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish, 594-608, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.