FIGURE

Fig. 2

ID

ZDB-FIG-061104-17

Publication

Sakaguchi et al., 2006 - The yolk syncytial layer regulates myocardial migration by influencing extracellular matrix assembly in zebrafish

Other Figures

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Fig. 2

Specificity of mtx1 MOs. (A) Schematic diagram of mtx1 genomic structure and position of mtx1 MOs. mtx1 has three exons (blue boxes) and two introns. Two independent ATG MOs, MO(A) and MO(B), and a splice donor MO, MO(C), caused essentially the same phenotype when injected into the YSL at the 1000-cell stage. The splice donor MO, mtx1 MO(C), leads to a 20 bp insertion in mtx1 mRNA, which leads to a frame shift and premature termination. Green arrowheads indicate the position of the primers used for the RT-PCR analysis shown in B. (B) RT-PCR analysis shows that an additional 20 bp are integrated into mtx1 mRNA in mtx1 MO(C)-injected embryos.

Expression Data

Gene:	mxtx1
Fish:	WT WT + MO3-mxtx1
Knockdown Reagent:	MO3-mxtx1
Anatomical Term:	whole organism
Stage:	Shield

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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