FIGURE
Fig. 10
Fig. 10
Knockdown of Robo3var2 dorsalizes embryos. Embryos were injected at the 1–2 cell stage with control or robo3var2 MO and processed for RNA in situ hybridization. Embryos were fixed at 70% epiboly and processed for ntl (A, B) and gsc (C, D) in 4 mm control (A, C) and robo3var2 (B, D) MO injected embryos. Embryos were fixed at 14 hpf and processed for scl (E, F) and pax2 (G, H; asterisks denote head expression) in control (E, G) and robo3var2 (F, H) MO injected embryos. Data for gsc (I) and (J) scl were quantitated. Twenty to 70 embryos were scored from at least two separate injection experiments. |
Expression Data
| Genes: | |
|---|---|
| Fish: | |
| Knockdown Reagent: | |
| Anatomical Term: | |
| Stage Range: | Shield to 10-13 somites |
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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Reprinted from Mechanisms of Development, 122(10), Challa, A.K., McWhorter, M.L., Wang, C., Seeger, M.A., and Beattie, C.E., Robo3 isoforms have distinct roles during zebrafish development, 1073-1086, Copyright (2005) with permission from Elsevier. Full text @ Mech. Dev.