FIGURE

Fig. 2

ID

ZDB-FIG-050831-3

Publication

Langenau et al., 2005 - Cre/lox-regulated transgenic zebrafish model with conditional myc-induced T cell acute lymphoblastic leukemia

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Fig. 2

Leukemic lymphoblasts express T cell markers and both scl and lmo2.(A) Semiquantitative RT-PCR showing that Myc-induced leukemias are arrested at a stage in which scl and lmo2 are coexpressed. RT-PCR analysis of FACS-sorted, GFP-labeled thymocytes from 70-day-old transgenic lck-GFP (lck+) and rag2-GFP (rag2+) fish or leukemic lymphoblasts isolated from diseased fish (denoted by numbers). RT, reverse transcription reactions; No RT, no reverse transcription controls. (B-M) RNA in situ hybridization of paraffin-embedded sections confirms that scl and lmo2 are coexpressed in lymphoblasts from rag2-EGFP-mMyc transgenic fish (shown here infiltrating the skeletal musculature) and that scl and lmo2 are expressed in a subset of thymocytes in 70-day-old, nonleukemic, lck-GFP transgenic zebrafish. Images are photographed at 400x (B-E), 50x (F-I), and 200x (J-M). Images in J-M are higher-magnification images of the respective regions in F-I (indicated by the boxed region in F).

Expression Data

Genes:	lmo2 tal1
Fish:	cz1Tg
Anatomical Term:	pro-T cell
Stage:	Days 45-89

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA