FIGURE

Fig. 5

ID

ZDB-FIG-050812-9

Publication

Sadler et al., 2005 - A genetic screen in zebrafish identifies the mutants vps18, nf2 and foie gras as models of liver disease

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Fig. 5

Mutation of vps18 results in vesicle accumulation and lysosome depletion in hepatocytes. (A) hi^2499A mutants and vps18 morphants develop hepatomegaly. Scale bar: 100 µm. (B-D) Hematoxylin and Eosin staining of day 5 wild-type (B) and vps18 mutant (C,D) livers. Hepatocytes in mutant embryos are large and accumulate vacuoles (arrows in D). Boxed area in C is enlarged in D. Scale bar: 5 µm. (E,F) TEM of day 5 wild-type (E) and vps18 (F) hepatocytes shows large, membrane-bound structures proteinacious material and debris, indicative of vacuoles (v) in the mutants. n, nucleus. (G) vps18 mutation reduces the number of lysosomes in hepatocytes. Error bars indicate standard error of the mean.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-vps18 vps18^hi2499aTg
Knockdown Reagent:	MO1-vps18
Observed In:	hepatocyte lysosome hepatocyte vacuole liver
Stage:	Day 5

Phenotype Detail

Acknowledgments

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