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wnt3a and wnt8 coordinately regulate tail development. (A-C) Wild-type development. (D-L) Embryos from a transgenic TOPdGFP line were injected with wnt3a MOs (D,E,F), wnt8 MOs (G,H,I) or both (J,K,L). Expression of the ß-catenin-responsive reporter was examined at bud stage by in situ hybridization with a probe for GFP (A,D,G,J). Embryos are shown in a dorsal view of the tailbud, with anterior to the top. At the 10-somite stage (B,E,H,K), TOPdGFP expression was again assessed by in situ hybridization, in this case with a Fast Red color reaction. myoD expression, to visualize the defect in tail development, is in blue. Embryos are flat mounted and shown in a dorsal view with anterior to the left. At 24 hpf (C,F,I,L), living embryos of each type are shown in a lateral view, anterior to the left.
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