FIGURE

Fig. 3

ID

ZDB-FIG-050304-2

Publication

Iovine et al., 2005 - Mutations in connexin43 (GJA1) perturb bone growth in zebrafish fins

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Fig. 3

Expression of connexin 43 in embryos. In situ hybridization was completed using a cx43 probe against the cx43 3′ UTR. (A) Expression of cx43 in wild-type embryo at 24 hpf. e, eyes; c, cerebellum; v, vasculature. (B) Expression of cx43 in sof embryo at 24 hpf. e, eyes; c, cerebellum; v, vasculature. (C) Expression of cx43 in wild-type larvae at 72 hpf. Embryos were treated with PTU to prevent the production of melanin and facilitate the identification of stained structures. l, lens epithelium; h, hindbrain; t and arrowhead, thymus. (D) RT-PCR from 72 hpf whole embryos (lane 1), 72 hpf embryonic hearts (lane 2), and adult hearts (lane 3). The top panel shows amplification from cx43-3′ UTR primers, the bottom panel shows amplification from col-1a primers. Approximate sizes of amplified products are shown on the right (arrowhead).

Expression Data

Gene:	gja1b
Fish:	WT gja1b^b123 (AB)
Anatomical Terms:	blood vasculature cerebellum heart hindbrain immature eye lens thymus thymus primordium whole organism
Stage Range:	Prim-5 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 278(1), Iovine, M.K., Higgins, E.P., Hindes, A., Coblitz, B., and Johnson, S.L., Mutations in connexin43 (GJA1) perturb bone growth in zebrafish fins, 208-219, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.