FIGURE

Fig. 3

ID

ZDB-FIG-050225-5

Publication

Schäfer et al., 2005 - Hedgehog and retinoid signalling confines nkx2.2b expression to the lateral floor plate of the zebrafish trunk

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Fig. 3

Differences in the expression patterns of nkx2.2a and nkx2.2b. nkx2.2a (A) and nkx2.2b (B) expression in embryos at the 18s stage. (C-F) Higher magnification of rostral (C,D) and caudal (E,F) head regions showing nkx2.2a (C,E) and nkx2.2b (E,F) expression in the ventral brain. Note differences in expression in the mid-diencephalic boundary (MDB), the mid-hindbrain boundary (MHB) and in the pancreatic progenitor region (pc). (G-J) Transversal sections showing expression of nkx2.2a (G,I) and nkx2.2b (H,J) in the ventral CNS at hindbrain (G,H) and trunk (I,J) levels (positions of sections indicated in A,B). (K,L) Gradual reduction of nkx2.2a (K), but maintenance of strong nkx2.2b expression (L) in the posterior trunk. (M-S) Transversal sections through the spinal cord of 18s wild-type embryos showing expression of foxa2 (M), nkx2.2b (blue) and shh (red) (N), tal2 (O), olig2 (red) and nkx2.2b (blue) (P), isl1 (Q), isl1 and nkx2.2b (R) at mid-trunk level, and pax6 at posterior trunk level (S). LFP, lateral floor plate; MDB, mid-diencephalic boundary; MFP, medial floor plate; MHB, midbrain-hindbrain boundary; mn, motoneurons; nc, notochord; pc, pancreatic progenitor cells; sn, sensory neurons. (A-F) lateral views and (K,L) dorsal views, anterior to the left. Scale bar in (B) represents 200 µm (for A,B), in (D) 100 µm (for C,D), in (F) 50 µm (for E,F), in (L) 30 µm (for K,L), and in (S) 20 µm (for G,I,H,J,M-S).

Expression Data

Genes:	isl1a nkx2.2a nkx2.2b shha tal2
Fish:	WT
Anatomical Terms:	brain lateral floor plate medial floor plate midbrain hindbrain boundary motor neuron neural tube pancreas primordium trunk
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Mechanisms of Development, 122(1), Schäfer, M., Kinzel, D., Neuner, C., Schartl, M., Volff, J.N., and Winkler, C., Hedgehog and retinoid signalling confines nkx2.2b expression to the lateral floor plate of the zebrafish trunk, 43-56, Copyright (2005) with permission from Elsevier. Full text @ Mech. Dev.