Fig. 5

gli1 knockdown phenocopies dtr/gli1. (A) Expression of nk2.2 is unaffected by injection of a control morpholino (MO). (B) dtr/gli1 mutations eliminate nk2.2 expression in some regions of the brain (arrow and arrowhead). (C) Injection of a gli1MO into wild-type embryos leads to a loss of nk2.2 expression identical to that seen in dtr/gli1 mutant embryos (compare arrows, see also Table 1). (D) Expression of fkd4 in the medial and lateral floor plate is unaffected by control MO injections. (E) fkd4 expression is extremely reduced in dtr/gli1 mutants (compare bracket and arrow to those in D). (F) fkd4 expression is similarly reduced in lateral floor plate cells after gli1 MO injection (compare bracket and arrow to those in D). (d′, e′, and f′) show cross sections through the trunk at the level of the yolk plug. nc; notochord