CRISPR

CRISPR2-sirt1

ID

ZDB-CRISPR-230921-1

Name

CRISPR2-sirt1

Previous Names

None

Target

Sequence

5' - GGACGAGAAACCGGCGCGGA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site is "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
wcm19	sirt1
wcm21	sirt1

Expression

Gene expression in Wild Types + CRISPR2-sirt1

No data available

Phenotype

Phenotype resulting from CRISPR2-sirt1

No data available

Phenotype of all Fish created by or utilizing CRISPR2-sirt1

Phenotype	Fish	Conditions	Figures
blastema fin regeneration decreased rate of continuous process, abnormal	sirt1^wcm19/wcm19 (AB/TU)	chemical treatment by environment: nicotinamide, amputation: caudal fin	Fig. S2 from Lin et al., 2021
blastema fin regeneration decreased rate of continuous process, abnormal	sirt1^wcm19/wcm19 (AB/TU)	chemical treatment by environment: nicotinamide, chemical treatment by environment: sirtinol, amputation: caudal fin	Fig. S2 from Lin et al., 2021
blastema fin regeneration decreased rate of continuous process, abnormal	sirt1^wcm21/wcm21 (AB/TU)	chemical treatment by environment: doxycycline, amputation: caudal fin	Fig. 4 from Lin et al., 2021
blastema fin regeneration decreased rate of continuous process, abnormal	sirt1^wcm21/wcm21 (AB/TU)	chemical treatment by environment: nicotinamide, amputation: caudal fin	Fig. 3 from Lin et al., 2021
blastema fin regeneration decreased rate of continuous process, abnormal	sirt1^wcm21/wcm21 (AB/TU)	chemical treatment by environment: nicotinamide, amputation: caudal fin	Fig. 4 from Lin et al., 2021

Citations