CRISPR

CRISPR1-lamb1a

ID

ZDB-CRISPR-230623-1

Name

CRISPR1-lamb1a

Previous Names

None

Target

lamb1a (1)

Sequence

5' - GGATCCTCAATCCTGAAGGC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
sh589	lamb1a
sh590	lamb1a

Expression

Gene expression in Wild Types + CRISPR1-lamb1a

No data available

Phenotype

Phenotype resulting from CRISPR1-lamb1a

No data available

Phenotype of all Fish created by or utilizing CRISPR1-lamb1a

Phenotype	Fish	Conditions	Figures
cardiac ventricle myh7l expression increased amount, abnormal	lamb1a^sh590/sh590	standard conditions	Fig. 3 from Derrick et al., 2021
endocardium aldh1a2 expression increased distribution, abnormal	lamb1a^sh590/sh590	standard conditions	Fig. 7 from Derrick et al., 2021
atrial endocardium klf2a expression increased amount, abnormal	lamb1a^sh590/sh590	standard conditions	Fig. 5 from Derrick et al., 2021
atrium myh6 expression increased amount, abnormal	lamb1a^sh590/sh590	standard conditions	Fig. 3 from Derrick et al., 2021
endocardium lamb1b expression mislocalised, abnormal	lamb1a^sh590/sh590	standard conditions	Fig. 5 from Derrick et al., 2021
endocardium lamb1b expression increased distribution, abnormal	lamb1a^sh590/sh590	standard conditions	Fig. 5 from Derrick et al., 2021
heart myl7 expression increased amount, abnormal	lamb1a^sh590/sh590	standard conditions	Fig. 2 , Fig. 6 from Derrick et al., 2021
endocardium aldh1a2 expression mislocalised, abnormal	lamb1a^sh590/sh590	standard conditions	Fig. 7 from Derrick et al., 2021
ventricular endocardium klf2a expression increased amount, abnormal	lamb1a^sh590/sh590	standard conditions	Fig. 5 from Derrick et al., 2021
heart myl7 expression spatial pattern, abnormal	lamb1a^sh590/sh590	standard conditions	Fig. 2 from Derrick et al., 2021
atrium cell DsRed2 expression increased amount, abnormal	lamb1a^sh590/sh590; f2Tg	standard conditions	Fig. 3 from Derrick et al., 2021
heart proximal side EGFP expression increased amount, abnormal	lamb1a^sh590/sh590; f2Tg; twu34Tg	standard conditions	Fig. 4 from Derrick et al., 2021
atrial endocardium DsRed2 expression increased amount, abnormal	lamb1a^sh590/sh590; f2Tg; twu34Tg	standard conditions	Fig. 6 from Derrick et al., 2021
heart distal side EGFP expression increased amount, abnormal	lamb1a^sh590/sh590; f2Tg; twu34Tg	standard conditions	Fig. 4 from Derrick et al., 2021
heart proximal side DsRed2 expression increased amount, abnormal	lamb1a^sh590/sh590; f2Tg; twu34Tg	standard conditions	Fig. 4 from Derrick et al., 2021
regulation of secondary heart field cardioblast proliferation increased process quality, abnormal	lamb1a^sh590/sh590; f2Tg; twu34Tg	standard conditions	Fig. 4 , Fig. 6 from Derrick et al., 2021
atrial endocardium EGFP expression increased amount, abnormal	lamb1a^sh590/sh590; f2Tg; twu34Tg	standard conditions	Fig. 6 from Derrick et al., 2021
heart distal side DsRed2 expression increased amount, abnormal	lamb1a^sh590/sh590; f2Tg; twu34Tg	standard conditions	Fig. 4 from Derrick et al., 2021
atrium DsRed2 expression increased amount, abnormal	lamb1a^sh590/sh590; f2Tg; twu34Tg	standard conditions	Fig. 4 from Derrick et al., 2021
atrium EGFP expression increased amount, abnormal	lamb1a^sh590/sh590; f2Tg; twu34Tg	standard conditions	Fig. 4 from Derrick et al., 2021
ventricular endocardium lamb1b expression decreased amount, abnormal	lamb1a^sh590/+ + MO1-tnnt2a + MO4-tp53	standard conditions	Fig. 5 from Derrick et al., 2021
endocardium klf2a expression decreased amount, abnormal	lamb1a^sh590/+ + MO1-tnnt2a + MO4-tp53	standard conditions	Fig. 5 from Derrick et al., 2021
atrial endocardium lamb1b expression decreased amount, abnormal	lamb1a^sh590/+ + MO1-tnnt2a + MO4-tp53	standard conditions	Fig. 5 from Derrick et al., 2021
endocardium klf2a expression amount, ameliorated	lamb1a^sh590/sh590 + MO1-tnnt2a + MO4-tp53	standard conditions	Fig. 5 from Derrick et al., 2021
ventricular endocardium lamb1b expression decreased amount, abnormal	lamb1a^sh590/sh590 + MO1-tnnt2a + MO4-tp53	standard conditions	Fig. 5 from Derrick et al., 2021
heart myl7 expression amount, ameliorated	lamb1a^sh590/sh590 + MO1-tnnt2a + MO4-tp53	standard conditions	Fig. 6 from Derrick et al., 2021
endocardium aldh1a2 expression amount, ameliorated	lamb1a^sh590/sh590 + MO1-tnnt2a + MO4-tp53	standard conditions	Fig. 7 from Derrick et al., 2021
atrial endocardium lamb1b expression decreased amount, abnormal	lamb1a^sh590/sh590 + MO1-tnnt2a + MO4-tp53	standard conditions	Fig. 5 from Derrick et al., 2021
heart myl7 expression increased amount, abnormal	lamb1a^sh590/sh590 + MO4-tp53	standard conditions	Fig. 6 from Derrick et al., 2021
endocardium lamb1b expression increased distribution, abnormal	lamb1a^sh590/sh590 + MO4-tp53	standard conditions	Fig. 5 from Derrick et al., 2021
endocardium aldh1a2 expression mislocalised, abnormal	lamb1a^sh590/sh590 + MO4-tp53	standard conditions	Fig. 7 from Derrick et al., 2021
atrial endocardium klf2a expression increased amount, abnormal	lamb1a^sh590/sh590 + MO4-tp53	standard conditions	Fig. 5 from Derrick et al., 2021
ventricular endocardium klf2a expression increased amount, abnormal	lamb1a^sh590/sh590 + MO4-tp53	standard conditions	Fig. 5 from Derrick et al., 2021
endocardium lamb1b expression mislocalised, abnormal	lamb1a^sh590/sh590 + MO4-tp53	standard conditions	Fig. 5 from Derrick et al., 2021
endocardium aldh1a2 expression increased distribution, abnormal	lamb1a^sh590/sh590 + MO4-tp53	standard conditions	Fig. 7 from Derrick et al., 2021
regulation of secondary heart field cardioblast proliferation normal process quality, ameliorated	lamb1a^sh590/sh590; f2Tg; twu34Tg + MO1-tnnt2a + MO4-tp53	standard conditions	Fig. 6 from Derrick et al., 2021
atrial endocardium DsRed2 expression amount, ameliorated	lamb1a^sh590/sh590; f2Tg; twu34Tg + MO1-tnnt2a + MO4-tp53	standard conditions	Fig. 6 from Derrick et al., 2021
atrial endocardium EGFP expression amount, ameliorated	lamb1a^sh590/sh590; f2Tg; twu34Tg + MO1-tnnt2a + MO4-tp53	standard conditions	Fig. 6 from Derrick et al., 2021
atrial endocardium EGFP expression increased amount, abnormal	lamb1a^sh590/sh590; f2Tg; twu34Tg + MO4-tp53	standard conditions	Fig. 6 from Derrick et al., 2021
regulation of secondary heart field cardioblast proliferation increased process quality, abnormal	lamb1a^sh590/sh590; f2Tg; twu34Tg + MO4-tp53	standard conditions	Fig. 6 from Derrick et al., 2021
atrial endocardium DsRed2 expression increased amount, abnormal	lamb1a^sh590/sh590; f2Tg; twu34Tg + MO4-tp53	standard conditions	Fig. 6 from Derrick et al., 2021

Citations