CRISPR

CRISPR1-ercc2

ID
ZDB-CRISPR-230228-1
Name
CRISPR1-ercc2
Previous Names
None
Target
Sequence
5' - GGTGTTCCTGGATCCAGCGGGG - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "GGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
whu312 ercc2
Expression
Gene expression in Wild Types + CRISPR1-ercc2
No data available
Phenotype
Phenotype resulting from CRISPR1-ercc2
No data available
Phenotype of all Fish created by or utilizing CRISPR1-ercc2
Phenotype Fish Conditions Figures
pancreas prss1 expression decreased distribution, abnormal ercc2whu312/whu312 standard conditions Fig. 3 with image from Ma et al., 2022
intestinal epithelial cell ab4-h3 labeling decreased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 4 with image from Ma et al., 2022
intestine morphology, abnormal ercc2whu312/whu312 standard conditions Fig. 1 with image from Ma et al., 2022
intestine has fewer parts of type intestine goblet cell, abnormal ercc2whu312/whu312 standard conditions Fig. 4 with image from Ma et al., 2022
swim bladder uninflated, abnormal ercc2whu312/whu312 standard conditions Fig. 7 with image from Ma et al., 2022
intestine ab3-map1lc3b labeling decreased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 5 with image from Ma et al., 2022
intestinal epithelial cell ribosome biogenesis decreased process quality, abnormal ercc2whu312/whu312 standard conditions Fig. 5 with image from Ma et al., 2022
mandibular arch skeleton malformed, abnormal ercc2whu312/whu312 standard conditions Fig. 1 with image from Ma et al., 2022
intestinal bulb fabp2 expression decreased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 2 with image from Ma et al., 2022
intestine decreased size, abnormal ercc2whu312/whu312 standard conditions Fig. 1 with image from Ma et al., 2022
intestine goblet cell decreased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 4 with image from Ma et al., 2022
intestine fabp2 expression decreased distribution, abnormal ercc2whu312/whu312 standard conditions Fig. 3 with image from Ma et al., 2022
whole organism mdm2 expression increased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 7 with image from Ma et al., 2022
pancreas decreased size, abnormal ercc2whu312/whu312 standard conditions Fig. 1 with image from Ma et al., 2022
exocrine pancreas prss1 expression decreased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 2 with image from Ma et al., 2022
swim bladder uninflated, abnormal ercc2whu312/whu312 standard conditions Fig. 1 with image from Ma et al., 2022
intestinal epithelial cell mitochondrion malformed, abnormal ercc2whu312/whu312 standard conditions Fig. 5 with image from Ma et al., 2022
digestive system fabp2 expression decreased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 7 with image from Ma et al., 2022
intestine lumen decreased size, abnormal ercc2whu312/whu312 standard conditions Fig. 2 with image from Ma et al., 2022
intestinal epithelial cell nucleus increased size, abnormal ercc2whu312/whu312 standard conditions Fig. 6 with image from Ma et al., 2022
yolk absorption quality, abnormal ercc2whu312/whu312 standard conditions Fig. 1 with image from Ma et al., 2022
pancreas prss1 expression decreased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 3 with image from Ma et al., 2022
liver decreased size, abnormal ercc2whu312/whu312 standard conditions Fig. 1 with image from Ma et al., 2022
whole organism il1b expression increased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 5 with image from Ma et al., 2022
intestine Ab1-mt-co1 labeling spatial pattern, abnormal ercc2whu312/whu312 standard conditions Fig. 5 with image from Ma et al., 2022
pancreas morphology, abnormal ercc2whu312/whu312 standard conditions Fig. 1 with image from Ma et al., 2022
whole organism mmp9 expression increased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 5 with image from Ma et al., 2022
intestine fabp2 expression decreased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 3 with image from Ma et al., 2022
intestinal epithelial cell nucleus ab1-fbl labeling increased distribution, abnormal ercc2whu312/whu312 standard conditions Fig. 6 with image from Ma et al., 2022
intestinal epithelial cell autophagy increased process quality, abnormal ercc2whu312/whu312 standard conditions Fig. 5 with image from Ma et al., 2022
mid intestine fabp6 expression decreased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 2 with image from Ma et al., 2022
whole organism saa expression increased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 5 with image from Ma et al., 2022
intestine Ab1-mt-co1 labeling decreased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 5 with image from Ma et al., 2022
liver fabp10a expression decreased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 2 with image from Ma et al., 2022
liver morphology, abnormal ercc2whu312/whu312 standard conditions Fig. 1 with image from Ma et al., 2022
intestinal epithelial cell nucleus ab1-fbl labeling increased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 6 with image from Ma et al., 2022
digestive system hbae3 expression decreased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 7 with image from Ma et al., 2022
intestinal epithelial cell nucleoplasm ab1-ncl labeling mislocalised, abnormal ercc2whu312/whu312 standard conditions Fig. 6 with image from Ma et al., 2022
eye decreased size, abnormal ercc2whu312/whu312 standard conditions Fig. 1 with image from Ma et al., 2022
liver increased accumulation liver lipid droplet, abnormal ercc2whu312/whu312 standard conditions Fig. 2 with image from Ma et al., 2022
gut collapsed, abnormal ercc2whu312/whu312 standard conditions Fig. 1 with image from Ma et al., 2022
whole organism curved, abnormal ercc2whu312/whu312 standard conditions Fig. 1 with image from Ma et al., 2022
intestine ab3-map1lc3b labeling spatial pattern, abnormal ercc2whu312/whu312 standard conditions Fig. 5 with image from Ma et al., 2022
whole organism viability, abnormal ercc2whu312/whu312 standard conditions Fig. 1 with image from Ma et al., 2022
intestinal epithelial cell nucleoplasm ab1-fbl labeling mislocalised, abnormal ercc2whu312/whu312 standard conditions Fig. 6 with image from Ma et al., 2022
whole organism cxcl8b.1 expression increased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 5 with image from Ma et al., 2022
whole organism mmp13a.1 expression increased amount, abnormal ercc2whu312/whu312 standard conditions Fig. 5 with image from Ma et al., 2022
intestinal epithelial cell inflammatory response increased process quality, abnormal ercc2whu312/whu312 standard conditions Fig. 5 with image from Ma et al., 2022
liver DsRed expression decreased amount, abnormal ercc2whu312/+; gz15Tg standard conditions Fig. 1 with image from Ma et al., 2022
liver DsRed expression decreased distribution, abnormal ercc2whu312/+; gz15Tg standard conditions Fig. 1 with image from Ma et al., 2022
pancreas DsRed expression decreased distribution, abnormal ercc2whu312/+; gz15Tg standard conditions Fig. 1 with image from Ma et al., 2022
pancreas DsRed expression decreased amount, abnormal ercc2whu312/+; gz15Tg standard conditions Fig. 1 with image from Ma et al., 2022
pancreas ela3l expression decreased distribution, abnormal ercc2whu312/whu312; gz15Tg standard conditions Fig. 3 with image from Ma et al., 2022
liver DsRed expression decreased amount, abnormal ercc2whu312/whu312; gz15Tg standard conditions Fig. 3 with image from Ma et al., 2022
pancreas ela3l expression decreased amount, abnormal ercc2whu312/whu312; gz15Tg standard conditions Fig. 3 with image from Ma et al., 2022
liver DsRed expression decreased distribution, abnormal ercc2whu312/whu312; gz15Tg standard conditions Fig. 3 with image from Ma et al., 2022
liver hypoplastic, abnormal ercc2whu312/whu312; gz15Tg standard conditions Fig. 1 with image from Ma et al., 2022
exocrine pancreas hypoplastic, abnormal ercc2whu312/whu312; gz15Tg standard conditions Fig. 1 with image from Ma et al., 2022
intestine endocrine cell EGFP expression mislocalised, abnormal ercc2whu312/whu312; ia3Tg standard conditions Fig. 4 with image from Ma et al., 2022
intestine endocrine cell nkx2.2a expression mislocalised, abnormal ercc2whu312/whu312; ia3Tg standard conditions Fig. 4 with image from Ma et al., 2022
intestine EGFP expression spatial pattern, abnormal ercc2whu312/whu312; sqet33J1Et standard conditions Fig. 1 with image from Ma et al., 2022
intestine EGFP expression decreased distribution, abnormal ercc2whu312/whu312; sqet33J1Et standard conditions Fig. 1 with image from Ma et al., 2022
gut decreased size, abnormal ercc2whu312/whu312; sqet33J1Et standard conditions Fig. 1 with image from Ma et al., 2022
intestine EGFP expression decreased amount, abnormal ercc2whu312/whu312; sqet33J1Et standard conditions Fig. 1 with image from Ma et al., 2022
swim bladder uninflated, abnormal tp53zdf1/zdf1; ercc2whu312/whu312 standard conditions Fig. 7 with image from Ma et al., 2022
digestive system fabp2 expression decreased amount, abnormal tp53zdf1/zdf1; ercc2whu312/whu312 standard conditions Fig. 7 with image from Ma et al., 2022
digestive system hbae3 expression decreased amount, abnormal tp53zdf1/zdf1; ercc2whu312/whu312 standard conditions Fig. 7 with image from Ma et al., 2022
Citations
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