CRISPR

CRISPR1-pkmb

ID

ZDB-CRISPR-210223-1

Name

CRISPR1-pkmb

Previous Names

None

Target

Sequence

5' - TGTGGAACAGGGCGTGGACATGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
s718	pkmb

Expression

Gene expression in Wild Types + CRISPR1-pkmb

No data available

Phenotype

Phenotype resulting from CRISPR1-pkmb

No data available

Phenotype of all Fish created by or utilizing CRISPR1-pkmb

Phenotype	Fish	Conditions	Figures
cardiac muscle cell proliferation decreased occurrence, abnormal	pkma^s717/s717; pkmb^s718/+	cryoablation: cardiac ventricle	Fig. 2 from Fukuda et al., 2020
cardiac ventricle Fibrin increased area, abnormal	pkma^s717/s717; pkmb^s718/+	cryoablation: cardiac ventricle	Fig. 2 from Fukuda et al., 2020
cardiac ventricle Collagen increased area, abnormal	pkma^s717/s717; pkmb^s718/+	cryoablation: cardiac ventricle	Fig. 2 from Fukuda et al., 2020
intersegmental vessel morphology, abnormal	pkma^s717/s717; pkmb^s718/s718; s843Tg	standard conditions	Fig. 2 from Stone et al., 2018
intersegmental vessel disconnected, abnormal	pkma^s717/s717; pkmb^s718/s718; s843Tg	standard conditions	Fig. 2 from Stone et al., 2018
intersegmental vessel endothelial cell proliferation disrupted, abnormal	pkma^s717/s717; pkmb^s718/s718; s843Tg	standard conditions	Fig. S Movie 1 from Stone et al., 2018
intersegmental vessel endothelial cell migration disrupted, abnormal	pkma^s717/s717; pkmb^s718/s718; s843Tg	standard conditions	Fig. S Movie 1 from Stone et al., 2018

Citations