CRISPR

CRISPR1-hcfc1a

ID

ZDB-CRISPR-210125-1

Name

CRISPR1-hcfc1a

Previous Names

None

Target

hcfc1a (1)

Sequence

5' - GGTTCATACCAGCCGTTCGT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
co60	hcfc1a
co64	hcfc1a

Expression

Gene expression in Wild Types + CRISPR1-hcfc1a

No data available

Phenotype

Phenotype resulting from CRISPR1-hcfc1a

No data available

Phenotype of all Fish created by or utilizing CRISPR1-hcfc1a

Phenotype	Fish	Conditions	Figures
brain ccne1 expression decreased amount, abnormal	hcfc1a^co60/+	chemical treatment by environment: EC 2.7.1.137 (phosphatidylinositol 3-kinase) inhibitor	Fig. 9 from Castro et al., 2020
brain neuronal stem cell apoptotic, abnormal	hcfc1a^co60/+	standard conditions	Fig. 4 from Castro et al., 2020
brain neural precursor cell proliferation increased occurrence, abnormal	hcfc1a^co60/+	control	Fig. 9 from Castro et al., 2020
brain neuronal stem cell increased amount, abnormal	hcfc1a^co60/+	control	Fig. 3 , Fig. 4 , Fig. 8 , Fig. 9 from Castro et al., 2020
forebrain neuronal stem cell increased amount, abnormal	hcfc1a^co60/+	standard conditions	Fig. 2 from Castro et al., 2020
brain sox2 expression decreased amount, abnormal	hcfc1a^co60/+	chemical treatment by environment: EC 2.7.1.137 (phosphatidylinositol 3-kinase) inhibitor	Fig. 9 from Castro et al., 2020
brain glial cell gfap expression increased amount, abnormal	hcfc1a^co60/+	standard conditions	Fig. 5 from Castro et al., 2020
whole organism sox2 expression increased amount, abnormal	hcfc1a^co60/+	standard conditions	Fig. 2 from Castro et al., 2020
hindbrain neural precursor cell proliferation normal occurrence, ameliorated	hcfc1a^co60/+	chemical treatment by environment: EC 2.7.1.137 (phosphatidylinositol 3-kinase) inhibitor	Fig. 8 from Castro et al., 2020
midbrain neural precursor cell proliferation increased occurrence, abnormal	hcfc1a^co60/+	control	Fig. 4 , Fig. 8 from Castro et al., 2020
midbrain neuronal stem cell increased amount, abnormal	hcfc1a^co60/+	standard conditions	Fig. 2 from Castro et al., 2020
hindbrain neural precursor cell proliferation increased occurrence, abnormal	hcfc1a^co60/+	control	Fig. 4 , Fig. 8 from Castro et al., 2020
brain neuron elavl3 expression increased amount, abnormal	hcfc1a^co60/+	standard conditions	Fig. 5 from Castro et al., 2020
hindbrain cell proliferation in hindbrain increased occurrence, abnormal	hcfc1a^co60/+	standard conditions	Fig. 7 from Castro et al., 2020
midbrain neural precursor cell proliferation normal occurrence, ameliorated	hcfc1a^co60/+	chemical treatment by environment: EC 2.7.1.137 (phosphatidylinositol 3-kinase) inhibitor	Fig. 8 from Castro et al., 2020
brain ccne1 expression increased amount, abnormal	hcfc1a^co60/+	control	Fig. 9 from Castro et al., 2020
forebrain neural precursor cell proliferation normal occurrence, ameliorated	hcfc1a^co60/+	chemical treatment by environment: EC 2.7.1.137 (phosphatidylinositol 3-kinase) inhibitor	Fig. 8 from Castro et al., 2020
whole organism hcfc1a expression decreased amount, abnormal	hcfc1a^co60/+	standard conditions	Fig. 1 from Castro et al., 2020
forebrain neural precursor cell proliferation increased occurrence, abnormal	hcfc1a^co60/+	control	Fig. 8 from Castro et al., 2020
forebrain cell proliferation in forebrain increased occurrence, abnormal	hcfc1a^co60/+	standard conditions	Fig. 7 from Castro et al., 2020
whole organism asxl1 expression increased amount, abnormal	hcfc1a^co60/+	standard conditions	Fig. 7 from Castro et al., 2020
brain olig2 expression increased amount, abnormal	hcfc1a^co60/+	standard conditions	Fig. 5 from Castro et al., 2020
brain neural precursor cell proliferation normal occurrence, ameliorated	hcfc1a^co60/+	chemical treatment by environment: EC 2.7.1.137 (phosphatidylinositol 3-kinase) inhibitor	Fig. 9 from Castro et al., 2020
neuronal stem cell apoptotic process increased occurrence, abnormal	hcfc1a^co60/+	standard conditions	Fig. 4 from Castro et al., 2020
hindbrain neuronal stem cell increased amount, abnormal	hcfc1a^co60/+	standard conditions	Fig. 2 from Castro et al., 2020
brain neuronal stem cell normal amount, ameliorated	hcfc1a^co60/+	chemical treatment by environment: EC 2.7.1.137 (phosphatidylinositol 3-kinase) inhibitor	Fig. 9 from Castro et al., 2020
brain sox2 expression increased amount, abnormal	hcfc1a^co60/+	control	Fig. 9 from Castro et al., 2020
brain asxl1 expression increased amount, abnormal	hcfc1a^co60/+	control	Fig. 9 from Castro et al., 2020
midbrain cell proliferation in midbrain increased occurrence, abnormal	hcfc1a^co60/+	standard conditions	Fig. 7 from Castro et al., 2020
swimming decreased occurrence, abnormal	hcfc1a^co60/+	standard conditions	Fig. 10 from Castro et al., 2020
brain asxl1 expression decreased amount, abnormal	hcfc1a^co60/+	chemical treatment by environment: EC 2.7.1.137 (phosphatidylinositol 3-kinase) inhibitor	Fig. 9 from Castro et al., 2020
forebrain cell proliferation in forebrain normal occurrence, ameliorated	hcfc1a^co60/+ + MO2-asxl1	standard conditions	Fig. 7 from Castro et al., 2020
hindbrain cell proliferation in hindbrain normal occurrence, ameliorated	hcfc1a^co60/+ + MO2-asxl1	standard conditions	Fig. 7 from Castro et al., 2020
midbrain cell proliferation in midbrain normal occurrence, ameliorated	hcfc1a^co60/+ + MO2-asxl1	standard conditions	Fig. 7 from Castro et al., 2020

Citations