Regional plot of significant locus for genome-wide association of Rey Complex Figure Test Delayed Component. The gray horizontal line represents the threshold for genome-wide significant association (p = 5 × 10−8). rs75885813, corrected p = 3.83 × 10−9, was the top-ranked SNP. The other 4 significant SNPs were in high linkage disequilibrium with rs75885813 (r2 > 0.8). All significant SNPs were located within RBFOX1. SNP, single nucleotide polymorphism.

Bubble diagram of the pathway analysis of potentially associated variants for Rey Complex Figure Test Delayed Component. The p value increased from top to bottom. All the pathways displayed in the figure were significant (corrected p < .05). The color represents p value, while the size represents the number of associated genes enriched in the particular pathway. mRNA, messenger RNA.

rbfox1 is required for visual and acoustic habituation in larval zebrafish. (A) Schematic diagram of visual habituation. (B) Mean percentage of habituation during the visual habituation phase in the rbfox1 mutant. (C) Schematic diagram of acoustic habituation. (D) Mean percentage of habituation during the acoustic habituation phase in the rbfox1 mutant. (E) Mean percentage of habituation during the visual habituation phase in the rbfox1 MO-injected fish. (F) Mean percentage of habituation during the acoustic habituation phase in the rbfox1 MO-injected fish. The data are expressed as mean ± standard error. n = 48 larval fish per group. ∗p < .05 (paired t test), ∗∗p < .01. MO, morpholino antisense oligo; mut, mutant; WT, wild-type

Zebrafish rbfox1 regulates both presynaptic and postsynaptic functions. (A) Kyoto Encyclopedia of Genes and Genomes pathway enrichment of differentially expressed genes in rbfox1 morphant. (B) Gene Ontology enrichment of differentially expressed genes in rbfox1 morphant. All zebrafish larvae were collected at 5 days post fertilization, and then whole transcriptome deep sequencing was performed. HIF, hypoxia-inducible factor; GnRH, gonadotropin-releasing hormone; Num, number; TRP, transient receptor potential

Posttranscriptional regulation of snap25a by Rbfox1. (A) Acoustic and visual habituation defects in snap25a-4sg mutant, tests performed on 5 days post fertilization, n ≥ 48. (B) Changes of snap25a isoform ratios (exon5 alternative inclusion) in rbfox1 mutant. The data are expressed as mean ± standard error. n = 48 larval fish per group. ∗∗∗p < .001, ∗∗p < .01, ∗p < .05 (unpaired t test). 4gRNA, 4 guide RNA; mut, mutant; WT, wild-type