Fig. 1. Effect of gamma radiation on zebrafish telencephalon development (48 hpf and 72 hpf). The red arrow in the figure indicates apoptosis and an abnormal telencephalon structure.

Fig. 2. Effect of γ radiation on zebrafish morphological development. (A) Three typical developmental deformity indexes: tail curvature, spinal curvature, pericardial sac, and yolk sac enlargement. (B) Hatching rate, mortality, and deformity rate of zebrafish 72 hpf; * P < 0.05; * * P < 0.01.

Fig. 3. Effects of different doses of gamma radiation on the movement rhythm of zebrafish embryos (120 hpf).(A) Changes in zebrafish motion trajectories under different irradiation doses in 96 well plates. a-h represents the different irradiation dose groups. The color of the line segment represents the speed, the black line represents low speed movement, the green line represents medium speed movement, the red line represents high speed movement, and the length of the line segment represents the swimming distance of the fish. (B) Changes in the activity speed of juvenile zebrafish in each group. (C) Exercise cycle, motion cycle refers to the time corresponding to the two peaks of motion trajectory. (D) Amplitude, meaning the maximum distance from the equilibrium position. (E) Phase, which refers to the position at a specific time in the wave period; * P < 0.05.

Fig. 4. Effect of gamma radiation on mRNA expression of the main clock gene of zebrafish embryo (72 hpf), A: bmal1b, B: clock1a, C: per1b, D: per2, E: cry2, F: nr1d1, * P < 0.05.

Fig. 5. Effects of different doses of gamma radiation on the expression of clock protein in zebrafish embryos (72 hpf); blot chart and gray value analysis; * P < 0.05.

Fig. 6. Effects of different doses of gamma radiation on melatonin secretion in zebrafish embryos at different time points (72 hpf); * P < 0.05.

Fig. 7. Changes in melatonin secretion in a zebrafish embryo after different doses of gamma radiation (72 hpf).