- Title
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Radiation dose enhancement using gold nanoparticles with a diamond linear accelerator target: a multiple cell type analysis
- Authors
- Piccolo, O., Lincoln, J.D., Melong, N., Orr, B.C., Fernandez, N.R., Borsavage, J., Berman, J.N., Robar, J., Ha, M.N.
- Source
- Full text @ Sci. Rep.
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HNCCs labelled with GNPs show decreased in vitro colony forming potential 10 days post radiation. A colony formation assay (CFA) was used to determine the potential of GNP-labelled or unlabelled (NoNP) cancer cells to form colonies 10 days after radiation with the STB or DTB. ( |
GNP-mediated DTBR significantly decreased HNCC proliferation in vivo. Groups of 15–20 zebrafish larvae were used for each time point and treatment group, 50–100 cells were injected into the yolk sac of each fish, and the number of fluorescent cells was quantified ex vivo. Xenografted cells were quantified at baseline (0 days post radiation (dpr)) and 3 dpr after treatment with 8 Gy from the STB or DTB. Results are represented as fold change of the unirradiated control cells (No RT). ( |
GNP labelled Detroit-562 and HSC-3 HNCCs demonstrate increased fluorescence intensity (FI) of double stranded breaks (DSBs) with DTBR compared to NoNP cells with STBR. Cells were cultured in 6-well plates, irradiated with 8 Gy from the STB or DTB, then fixed 30 min after radiation. Cells were processed for immunohistochemistry (IHC) with γ-H2AX to assess DNA double strand breaks and DAPI nuclear stain. ( |
Reactive oxygen species (ROS) were elevated in HNCCs with GNP-facilitated DTBR. Cells were cultured in 6-well plates, irradiated with 8 Gy from the STB or DTB, then processed for flow cytometry 12 h post radiation. ( |