- Title
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Neuron-glia interaction through Serotonin-BDNF-NGFR axis enables regenerative neurogenesis in Alzheimer's model of adult zebrafish brain
- Authors
- Bhattarai, P., Cosacak, M.I., Mashkaryan, V., Demir, S., Popova, S.D., Govindarajan, N., Brandt, K., Zhang, Y., Chang, W., Ampatzis, K., Kizil, C.
- Source
- Full text @ PLoS Biol.
(A) Schematic view of the experimental pipeline for whole-transcriptome sequencing IL4 treatment. (B) MA-plot for DEGs. (C) GO-term analyses on DEGs. (D) Modified KEGG-pathway view of tryptophan metabolism. Green indicates the enzymes down-regulated by IL4. (E) IHC for 5-HT in control (left), Aβ42-injected (middle), and IL4-injected (right) brains. Single-channel images show 5-HT. Red insets are high-magnification images of arrowed regions. (Dm: dorsal-medial) (F) Quantification of 5-HT-span area density under the conditions of E. (G) qRT-PCR results for |
(A, B) IHC for S100β and PCNA in control (A) and 5-HT-injected (B) brains. (C) Quantification of proliferating glia in conditions of panels A and B. (D, E) IHC for BrdU and HuC/D for newborn neurons at 14 dpi after BrdU treatment at 2 and 3 dpi (D) and BDNF injection (E). (F) Quantification of newborn neurons. (G) IHC for GFP (driven by glial promoter her4.1) and PCNA in control, IL4-injected, Aβ42-injected, and 5-HT-injected brains. (H) Quantification of proliferating glia in conditions of panel G. (I-K) IHC for her4.1-driven GFP and 5-HT. The composite image (I) and single fluorescent channels for her4.1:GFP (J) and 5-HT (K). (L) ISH for EXPRESSION / LABELING:
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(A) Schematic workflow for single-cell sequencing and data analyses. (B) Distribution plot for DEGs in |
(A–C) ISH for EXPRESSION / LABELING:
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(A–A5) IHC for S100β and PCNA in control (A), control morpholino-injected (A1), |
Aβ42, amyloid-beta42; bdnf, brain-derived neurotrophic factor; htr1, 5-hydroxytryptamine receptor 1; IL4, interleukin-4; NFkB, nuclear factor 'kappa-light-chain-enhancer' of activated B-cells; NGFRA, nerve growth factor receptor A; NSC, neural stem cell; PCNA, proliferating cell nuclear antigen; pERK, hosphorylated extracellular signal-regulated kinase; pSTAT6, phosphorylated signal transducer and activator of transcription 6, interleukin-4 induced; PVZ, periventricular zone; ngfra, nerve growth factor receptor A; Tph2, tryptophan hydroxylase; 5-HT, serotonin. |