Fig 1

Fig 1

(A) Schematic view of the experimental pipeline for whole-transcriptome sequencing IL4 treatment. (B) MA-plot for DEGs. (C) GO-term analyses on DEGs. (D) Modified KEGG-pathway view of tryptophan metabolism. Green indicates the enzymes down-regulated by IL4. (E) IHC for 5-HT in control (left), Aβ42-injected (middle), and IL4-injected (right) brains. Single-channel images show 5-HT. Red insets are high-magnification images of arrowed regions. (Dm: dorsal-medial) (F) Quantification of 5-HT-span area density under the conditions of E. (G) qRT-PCR results for tph1a, tph1b, and tph2 in control, amyloid-injected, and IL4-injected zebrafish brains. Beta-actin used for normalization. n = 3 animals for experiments. Scale bars equal 100 μM. Data are represented as mean ± SEM. See also S1 Fig and S2 Fig. See S2 Data and S7 Data for supporting information. Aβ42, amyloid-beta42; DEG, differentially expressed gene; ECM, extracellular matrix; FC, fold change; FDR, false discovery rate; GFP, green fluorescent protein; GO, gene ontology; IHC, immunohistochemistry; KEGG, Kyoto Encyclopedia of Genes and Genomes; IL4, interleukin-4; MA, Bland-Altman mean-average plot; qRT-PCR, quantitative real-time polymerase chain reaction; 5-HT, serotonin.