ZFIN All Figures, Villasenor <i>et al.</i>, 2019

Fig. 1

hhex−/− display impaired liver and pancreas development. (A–C) Analysis of the endodermal phenotypes in hhex−/−. (A) Lateral views of 96 hpf Tg(fabp:GFP) hhex+/+ sibling and hhex−/− larvae. (B) Maximum intensity projections of 96 hpf hhex+/+ sibling and hhex−/− larvae. Ventral views, anterior to the top. hhex−/− display impaired liver development. (C) Dorsal views of whole-mount in situ hybridization for ptf1a expression at 32 and 72 hpf. Compared to stage-matched hhex+/+ siblings, hhex−/− lack exocrine tissue specification, but they display ptf1a neural expression. Black arrows point to pancreatic exocrine tissue. H, heart; L, liver; P, pancreas. Scale bars: 50 μm.

EXPRESSION / LABELING:

Gene:	ptf1a
Fish:	WT hhex^s721/s721
Anatomical Terms:	brain exocrine pancreas intestinal bulb primordium
Stage Range:	Prim-15 to Protruding-mouth

Expression / Labeling details

PHENOTYPE:

Fish:	hhex^s721/s721 hhex^s721/s721; as3Tg hhex^s721/s721; nim5Tg
Observed In:	exocrine pancreas intestinal bulb primordium liver pancreas
Stage Range:	Prim-15 to Day 4

Phenotype details

Fig. 2

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:

Gene:	hhex
Antibody:	Ab-2F11
Fish:	WT hhex^s721/s721
Anatomical Terms:	blood vessel endothelial cell common bile duct cystic duct digestive system duct endoderm extrahepatic duct extrapancreatic duct gall bladder intrahepatic bile duct liver posterior pancreatic bud primary islet
Stage Range:	20-25 somites to Protruding-mouth

Expression / Labeling details

PHENOTYPE:

Fish:	hhex^s721/s721 hhex^s721/s721; s870Tg
Observed In:	common bile duct cystic duct digestive system duct digestive system duct hepaticobiliary system development extrahepatic duct extrapancreatic duct gall bladder intestinal bulb
Stage Range:	Prim-15 to Protruding-mouth

Phenotype details

Fig. 3

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:

Genes:	prox1a sox9b
Fish:	WT hhex^s721/s721
Anatomical Terms:	anterior pancreatic bud central nervous system digestive system duct liver posterior pancreatic bud
Stage:	Prim-25

Expression / Labeling details

PHENOTYPE:

Fish:	hhex^s721/s721
Observed In:	anterior pancreatic bud digestive system duct liver
Stage:	Prim-25

Phenotype details

Fig. 4

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

PHENOTYPE:

Fish:	npas4l^s5/s5; s870Tg
Observed In:	digestive system duct digestive system duct hepaticobiliary system development
Stage:	Protruding-mouth

Phenotype details

Fig. 5

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

PHENOTYPE:

Fish:	s870Tg + MO1-hhex
Knockdown Reagent:	MO1-hhex
Observed In:	digestive system duct hepaticobiliary system development
Stage:	Long-pec

Phenotype details

Fig. S1

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

PHENOTYPE:

Fish:	hhex^s721/s721 hhex^s721/s721; gz19Tg
Observed In:	pancreatic A cell pancreatic B cell pancreatic D cell
Stage:	Protruding-mouth

Phenotype details

Fig. S4

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:

Antibody:	Ab-2F11
Fish:	hhex^s721/s721
Anatomical Term:	primary islet cell
Stage:	Day 5

Expression / Labeling details

PHENOTYPE:

Fish:	hhex^s721/s721
Observed In:	primary islet cell
Stage:	Day 5

Phenotype details

text only

Unillustrated author statements

PHENOTYPE:

Fish:	hhex^s721/s721 hhex^s722/s722
Observed In:	whole organism
Stage:	Days 7-13

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 458(2), Villasenor, A., Gauvrit, S., Collins, M.M., Maischein, H.M., Stainier, D.Y.R., Hhex regulates the specification and growth of the hepatopancreatic ductal system, 228-236, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.

Villasenor et al., 2019 ZDB-PUB-191108-32 PMID:31697936

Hhex regulates the specification and growth of the hepatopancreatic ductal system

Villasenor et al., 2019

ZDB-PUB-191108-32
PMID:31697936