dnmt3 and dnmt5 expression is enriched in the ciliary marginal zone. A,F: In situ hybridization shows dnmt3 and dnmt5 expression throughout the anterior region of the embryo at 24 hr postfertilization (hpf). B–D,G–I: Expression becomes restricted to the CMZ, tectum, pectoral fins, and pharyngeal arches between 36 and 72 hpf. E,J: At 4 days postfertilization (dpf), ocular expression is only detected in the CMZ. Transverse sections, dorsal up (K,L).

dnmt4 and dnmt7 expression in the lens epithelium and ciliary marginal zone. A–E,K: In situ hybridization shows broad dnmt4 expression in the developing eye at 24 hr postfertilization (hpf) (A), which later becomes localized in the CMZ and lens epithelium (B–E, K, arrow points to LE). dnmt4 expression is also observed in the tectum, developing pectoral fin, and pharyngeal arches (A–E). F: dnmt7 is expressed ubiquitously at 24 hpf. G–J,L: At 36 hpf, expression remains diffuse in the anterior part of the embryo, and surrounding of the eye, while expression within the eye is only observed in the CMZ and LE (L, arrow points to LE). Transverse sections, dorsal up (K,L).

dnmt6 and dnmt8 expression in lens epithelial (LE), ciliary marginal zone (CMZ), ganglion cell layer (GCL), and inner nuclear layer (INL). In situ hybridization shows similar expression patterns between dnmt6 and dnmt8. A,B,F,G: At 24–36 hr postfertilization (hpf), both genes are expressed ubiquitously throughout the anterior part of embryo. C,H,K,L: At 48 hpf, expression is detected specifically in LE and CMZ, and weakly in the developing GCL and INL. D,I,M,N: At 72 hpf, expression is only detected within the GCL and INL. E,J: At 4dpf, expression is faint in the GCL. K-N: Transverse sections, dorsal up.