- Title
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Combinatorial expression patterns of heparan sulfate sulfotransferases in zebrafish: I. The 3-O-sulfotransferase family
- Authors
- Cadwallader, A.B., and Yost, H.J.
- Source
- Full text @ Dev. Dyn.
Expression of heparan sulfate (HS) 3-O-sulfotransferases (3-OST) genes during early zebrafish development. A-C: Expression of 3-OST-1 is ubiquitous in 2 cell (A), 7 somite (B), and 18 somite (C) embryos. D-F: Expression of 3-OST-3X in 2 cell (D), 13 somite (E), and 18 somite (F) embryos. G-I: Expression of 3-OST-3Z in 2 cell (G), 7 somite (H), and 18 somite (I) embryos. J-L: Expression of 3-OST-4 in 2 cell (J), 5 somite (K), and 18 somite (L) embryos. M-O: Expression of 3-OST-5 in 2 cell (M), 7 somite (N), and 18 somite (O) embryos. P-R: Expression of 3-OST-6 in 2 cell (P), 13 somite (Q), and 18 somite (R) embryos. FB, forebrain; HB, hindbrain; LPM, lateral plate mesoderm; MB, midbrain; NT, neural tube; PND, pronephric duct; S, somites; SC, spinal cord; SCN, spinal cord neurons; TB, tail bud. EXPRESSION / LABELING:
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The 3-O-sulfotransferases (3-OST) family is spatially restricted at 24 hr postfertilization. A: 3-OST-1 is expressed throughout the head and in the anterior somites. B: 3-OST-2 is expressed basally in the brain and specifically in the otic vesicle. C: 3-OST-3X is expressed in the hindbrain, spinal cord, branchial arches, pectoral fin, and lateral line primordial. D: 3-OST-3Z is expressed in the brain, spinal cord, pectoral fin, lateral line primordial, proctodeum, and dorsal fin fold. E: 3-OST-4 is expressed specifically in spinal cord neurons. F: 3-OST-5 is expressed specifically in the olfactory region and in posterior spinal cord neurons. G: 3-OST-6 is expressed specifically in the hindbrain. H: 3-OST-7 is expressed basally throughout the embryo and specifically in the posterior somites. BA, branchial arches; DFF, dorsal fin fold; FB, forebrain; HB, hindbrain; LLP, lateral line primordial; MB, midbrain; OE, olfactory epithelium; OT, otic vesicle; PF, pectoral fin; S, somites; SC, spinal cord; SCN, spinal cord neurons. EXPRESSION / LABELING:
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Expression of 3-O-sulfotransferases (3-OST) family members in the brain. A,B: Lateral (A) and dorsal (B) expression of 3-OST-1 at 48 hours postfertilization (hpf). C,D: Lateral (C) and dorsal (D) expression of 3-OST-2 at 48 hpf. E,F: Lateral (E) and dorsal (F) expression of 3-OST-3X at 36 hpf. G,H: Lateral (G) and dorsal (H) expression of 3-OST-3Z at 48 hpf. I,J: Lateral (I) and dorsal (J) expression of 3-OST-4 in 36 hpf embryos. K-P: Expression of 3-OST-5 in 36 hpf (K-M; lateral [K], dorsal [L], and rostral [M]) and at 48 hpf (N-P; rostral [N], lateral [O], and dorsal [P]). Q,R: Lateral (Q) and dorsal (R) expression of 3-OST-6 in 36 hpf embryos. S,T: Lateral (S) and dorsal (T) expression of 3-OST-7 in 36 hpf embryos. BA, branchial arches; DI, diencephalon; FB, forebrain; HB, hindbrain; MB, midbrain; OE, olfactory epithelium; OT, otic vesicle; RGC, retinal ganglion cells; TE, telencephalon. |
Expression of 3-OST family members in the developing pectoral fin. A: Weak expression of 3-OST-1 is observed in the pectoral fin muscle (white arrowhead). B: Strong expression of 3-OST-3X is seen in the AER (black arrowhead). C: The 3-OST-3Z is strongly expressed in both the muscle and AER. D: The 3-OST-7 is expressed weakly in the fin muscle. EXPRESSION / LABELING:
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