Spatio-temporal distribution of zebrafish crabp2a mRNA during early embryonic development. Whole mount in situ hybridization showing zebrafish crabp2a mRNA in the embryos during 80% epiboly at approximately 9 hpf (A, B), then during late gastrula at approximately 10 hpf (C, D), during early and middle somitogenesis at approximately 11 hpf (E, F) and at approximately 17 hpf (G–O). (A, C) Dorsal view, anterior is to the top; (B, D) side view, anterior is to the top; (E, G, H, L) dorsal view, anterior is to the left; (F, K, I, O) side view, anterior is to the left; (J) frontal view showing forebrain (Fb) and retina (Re), dorsal is to top; (M, N) transverse, dorsal is to top. The crabp2a mRNA expression in adaxial cells (Ac); adaxial epiblast (Ae); adaxial neuroectoderm (An); chordo-neural hinge (Cnh); diencephalon (Di); epidermis (Ep); floor plate (Fp); hindbrain (Hb); notochord (No); neural plate border (Npb); posterior epiblast (Pe); posterior epidermis (PEp); posterior neural plate (Pnp); rhombomeres (r) 2, 4, 6 and 7; roof plate (Rp); spinal cord (Sc); somites (So); telencephalon (Te) and ventral spinal cord (VSo) is indicated.

Spatio-temporal distribution of zebrafish crabp2b mRNA during early embryonic development. Expression of zebrafish crabp2b mRNA was detected by whole mount in situ hybridization to the zebrafish embryos during early gastrula at approximately 6 hpf (A), during late gastrula at approximately 10 hpf (B), during early somitogenesis at approximately 11 hpf (C–G) and during middle somitogenesis at approximately 17 hpf (H, I). (A) side view, anterior is to the top; (B) dorsal view, anterior is to the top; (C) dorsal view, anterior is to the left; (D) dorsal view of head, anterior is to the left; (E, H) dorsal view of tail, anterior is to the left; (F) dorsal view of trunk, anterior is to the left; (G, I) side view, anterior is to the left. The crabp2b mRNA expression in anterior epidermis (Aep), blastoderm (Bd), epidermis (Ep), posterior neural plate (Pnp), posterior somites (Pso), presumptive anterior spinal cord (Pasc), somites (So), stripe at the level of hind brain (Sphb) and unsegmented paraxial mesoderm (Upm) is indicated.

Distribution of zebrafish crabp2a mRNA during pharyngula stage of embryonic development. Whole mount and lateromedial section in situ hybridization showing zebrafish crabp2a mRNA in embryos at 24 hpf (A–H), 36 hpf (I–M) and 48 hpf (N–U) stages. All results are from whole mount in situ except (L, M) and (N). (A, E, G, I, N, U) Dorsal view, anterior is to left; (B, C, F, H, J, K, P–R, T) side view, anterior is to left; (D) frontal, dorsal is to top; (O) frontal, anterior is to the left; (L, M, S) transverse section, dorsal is to the top. The crabp2a mRNA expression in branchial arches (Ba); cerebellum (Ce); chordo-neural hinge (Cnh); dorsal diencephalon (DDi); diencephalon (Di); dorsal hindbrain (Dhb); dorsal spinal cord (Dsc); forebrain (Fb); foor plate (Fp); hindbrain (Hb); midbrain (Mb); notochord (No); oesophagus (Oe); optic nerve (On); optic stalk (Os); pectoral fin (Pf); proliferative zone of retina (PZRe); rhombomeres (r) 2, 4 and 6; retina (Re); roof plate (Rp); spinal cord (Sc); somite(s) (So); telencephalon (Te); ventral mesoderm (Vm); ventral mesenchyme (VMe) and ventral somite (VSo) is indicated.

Distribution of zebrafish crabp2b mRNA during pharyngula stage of embryonic development. Whole mount in situ hybridization showing zebrafish crabp2b mRNA in embryos at 24 hpf (A–E), 36 hpf (F–I) and 48 hpf (J–L) stages. (A, B, D, f, G, I, J, L) Dorsal view, anterior is to left; (C, E, H, K) side view, anterior is to left. The crabp2b mRNA expression in the anterior dorsal spinal cord (Adsc), anterior spinal cord (Asc), branchial arches (Ba), caudal most somite(s) (Cms), otic vesicle (Ov), part of retina (Pre), pectoral fin (Pf), posterior branchial arch (Pba) and retina dorsal to lens (ReD) is indicated.

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