Genomic Feature
wcm2
Notes
| Comment | Citation |
|---|---|
| TALENS were designed to target exon 2 of the sphk2 gene directly upstream of ... | Mendelson et al., 2015 |
Variants
- Variant Type
- Delins
- Variant Location
- Chr 16: 32138052 - 32138057 (GRCz11) (1) Details
- Nucleotide change
- TCGACT/A
- Variant Notes
- None
Effect on DNA/cDNA, transcript, protein (from publications)
- DNA/cDNA Change
- 1 bp inserted / 5 bp deleted in Exon 2 (1)
- Transcript Consequence
- Premature Stop (1)
- Protein Consequence
- Polypeptide Truncation (1)
- Flanking Sequence
-
ACTAGAGAAAGATGAATTCAAAATAAGTGTCAACAGCTGAATAAAAGCAGCACAACTTTGCCCTATGTGGGAAGAAAGAAGTCTTTAGATAAAATCTAGGTGAAGATCAGGAATGTACATGCATTGTGCACTGGAAAGATAACGTATCTCTCAAATTCTCAAATCAAAATCTTAAAAACATGATGAATTGCTCTTAAGATATCAAACAATTCAACCTTTAATAAACCGTTTTATTGAATAAATGGTTAGCTGGATATCTTTAACTTGATCTTTTAAATTCTCAATTTATGAGGATTTTTAAAAATGAGGGTGAGAAATACGTGTATTTTTTGGGGTGCTTATTTTTATATCTGTTAAAAACACTCTATTTTCAGATCACTTCATCTGTTGCTTAGTGTTTGGCCTAGTGCTTACTTTGCCTAAGATTGAAGGCTTTACGCTCATTTGTTTTTCCATTTGTATTGGGCAGAATTTTGCAAAAGCATGTTGCCTCGTCCCCG
TCGACT/A GTTGCTGCTGGTCAATCCATTCAGTGGCCGGGGTCAGGCAATGCAATGGTGTCAAACGCACATACTTCCAATGATAAGAGAAGCTAACATCAGCTATAACCTCATACAGACAGGTAAGACCAAACATGTGCAATCTCACAAATAAGATGTCAGCTGTAAATGGCTCAGAATATAATTGATGTACACAAAGCAATAGTGGTCAGAATTGCACTGAACGGATGTGATTTTTGTTGTTGTGCAGAGCGTCAGAATCACGCACGTGAGCTAATCAGAGAGATCTCACTACCAGAATGGGATGGAATCGTCATTGTGTCTGGAGATGGACTTTTGCATGAGGTGGATATTTTCATCACACCTTGCATAATTATTTATTATAATGCTTCGATGAAATCAGTCAAATTTTTGCCAGTATTTGTTTTGTGTGAATTTTTATTGTAATCATTTTCAAAATGTCAAATGGCTAAGATAAACTTAGTAGAACAAATTTTTTAAAAATATAT - Additional Sequence
- None
Fish
| Fish | Genomic Feature Zygosity | Parental Zygosity | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|---|---|
| sphk2wcm2/wcm2 | Homozygous | ♀+/- ♂+/- | Fig. 7  from Mendelson et al., 2017 | Fig. 7 from Mendelson et al., 2017 | |
| sphk2wcm2/wcm2 | Homozygous | ♀-/- ♂-/- | 5 figures from 2 publications | 3 figures from 2 publications | |
| sphk2wcm2/wcm2 | Homozygous | ♀-/- ♂+/- | Fig. 3 from Mendelson et al., 2015 | Fig. 3 from Mendelson et al., 2015 | |
| sphk2wcm2/wcm2 | Homozygous | ♀+/- ♂-/- | Fig. 3 from Mendelson et al., 2015 | 2 figures from 2 publications | |
| sphk2wcm2/wcm2 (AB/TU) | Homozygous | ♀+/- ♂+/- | 2 figures from Park et al., 2019 | ||
| sphk2wcm2/+ | Heterozygous | ♀+/- ♂+/+ | Fig. 4 from Mendelson et al., 2017 | ||
| sphk2wcm2/+ | Heterozygous | ♀+/- ♂-/- | Fig. 3 from Mendelson et al., 2015 | Fig. 3 from Mendelson et al., 2015 | |
| sphk2wcm2/+ | Heterozygous | ♀-/- ♂+/- | Fig. 3 from Mendelson et al., 2015 | Fig. 3 from Mendelson et al., 2015 | |
| sphk2wcm2/+ | Heterozygous | ♀-/- ♂+/+ | 2 figures from 2 publications | Fig. 3 from Mendelson et al., 2015 | |
| cers2bwcm18/wcm18; sphk2wcm2/wcm2 | Complex |
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Supplemental Information
- Genotyping protocol
- None
- Park, K.H., Ye, Z.W., Zhang, J., Hammad, S.M., Townsend, D.M., Rockey, D.C., Kim, S.H. (2019) 3-ketodihydrosphingosine reductase mutation induces steatosis and hepatic injury in zebrafish. Scientific Reports. 9:1138
- Mendelson, K., Pandey, S., Hisano, Y., Carellini, F., Das, B., Hla, T., Evans, T. (2017) The ceramide synthase 2b gene mediates genomic sensing and regulation of sphingosine Levels during zebrafish embryogenesis. eLIFE. 6
- Mendelson, K., Lan, Y., Hla, T., Evans, T. (2015) Maternal or zygotic sphingosine kinase is required to regulate zebrafish cardiogenesis. Developmental Dynamics : an official publication of the American Association of Anatomists. 244(8):948-54
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