PUBLICATION

Effects of Inducible Nitric Oxide Synthase (iNOS) Gene Knockout on the Diversity, Composition, and Function of Gut Microbiota in Adult Zebrafish

Authors
Huang, Y., Chen, Y., Xie, H., Feng, Y., Chen, S., Bao, B.
ID
ZDB-PUB-240627-20
Date
2024
Source
Biology   13(6): (Journal)
Registered Authors
Bao, Baolong
Keywords
16S rRNA, gut microbiota, immune, inducible nitric oxide synthase, zebrafish
MeSH Terms
none
PubMed
38927252 Full text @ Biology (Basel)
Abstract
The gut microbiota constitutes a complex ecosystem that has an important impact on host health. In this study, genetically engineered zebrafish with inducible nitric oxide synthase (iNOS or NOS2) knockout were used as a model to investigate the effects of nos2a/nos2b gene single knockout and nos2 gene double knockout on intestinal microbiome composition and function. Extensive 16S rRNA sequencing revealed substantial changes in microbial diversity and specific taxonomic abundances, yet it did not affect the functional structure of the intestinal tissues. Notably, iNOS-deficient zebrafish demonstrated a decrease in Vibrio species and an increase in Aeromonas species, with more pronounced effects observed in double knockouts. Further transcriptomic analysis of the gut in double iNOS knockout zebrafish indicated significant alterations in immune-related and metabolic pathways, including the complement and PPAR signaling pathways. These findings underscore the crucial interplay between host genetics and gut microbiota, indicating that iNOS plays a key role in modulating the gut microbial ecology, host immune system, and metabolic responses.
Genes / Markers
Marker Marker Type Name
adgrf6GENEadhesion G protein-coupled receptor F6
c3a.1GENEcomplement C3a, tandem duplicate 1
c5GENEcomplement component 5
c6.1GENEcomplement component 6, duplicate 1
c6.2GENEcomplement component 6, duplicate 2
c8gGENEcomplement component 8, gamma polypeptide
cd109GENECD109 molecule
cd28GENECD28 molecule
cd36GENECD36 molecule (CD36 blood group)
cfbGENEcomplement factor B
1 - 10 of 21
Show
Figures
Figure Gallery (6 images)
Show all Figures
Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
sou2
    		Small Deletion
    sou16
      		Small Deletion
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      nos2aCRISPR1-nos2aCRISPR
      1 - 1 of 1
      Show
      Fish
      No data available
      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      No data available
      Mapping
      No data available
      Your Input Welcome
      We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
      Citation
      Huang, Y., Chen, Y., Xie, H., Feng, Y., Chen, S., Bao, B. (2024) Effects of Inducible Nitric Oxide Synthase (iNOS) Gene Knockout on the Diversity, Composition, and Function of Gut Microbiota in Adult Zebrafish. Biology. 13(6):.