PUBLICATION
Effects of Inducible Nitric Oxide Synthase (iNOS) Gene Knockout on the Diversity, Composition, and Function of Gut Microbiota in Adult Zebrafish
- Authors
- Huang, Y., Chen, Y., Xie, H., Feng, Y., Chen, S., Bao, B.
- ID
- ZDB-PUB-240627-20
- Date
- 2024
- Source
- Biology 13(6): (Journal)
- Registered Authors
- Bao, Baolong
- Keywords
- 16S rRNA, gut microbiota, immune, inducible nitric oxide synthase, zebrafish
- MeSH Terms
- none
- PubMed
- 38927252 Full text @ Biology (Basel)
Citation
Huang, Y., Chen, Y., Xie, H., Feng, Y., Chen, S., Bao, B. (2024) Effects of Inducible Nitric Oxide Synthase (iNOS) Gene Knockout on the Diversity, Composition, and Function of Gut Microbiota in Adult Zebrafish. Biology. 13(6):.
Abstract
The gut microbiota constitutes a complex ecosystem that has an important impact on host health. In this study, genetically engineered zebrafish with inducible nitric oxide synthase (iNOS or NOS2) knockout were used as a model to investigate the effects of nos2a/nos2b gene single knockout and nos2 gene double knockout on intestinal microbiome composition and function. Extensive 16S rRNA sequencing revealed substantial changes in microbial diversity and specific taxonomic abundances, yet it did not affect the functional structure of the intestinal tissues. Notably, iNOS-deficient zebrafish demonstrated a decrease in Vibrio species and an increase in Aeromonas species, with more pronounced effects observed in double knockouts. Further transcriptomic analysis of the gut in double iNOS knockout zebrafish indicated significant alterations in immune-related and metabolic pathways, including the complement and PPAR signaling pathways. These findings underscore the crucial interplay between host genetics and gut microbiota, indicating that iNOS plays a key role in modulating the gut microbial ecology, host immune system, and metabolic responses.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping