PUBLICATION
Rbpms2 promotes female fate upstream of the nutrient sensing Gator2 complex component Mios
- Authors
- Wilson, M.L., Romano, S.N., Khatri, N., Aharon, D., Liu, Y., Kaufman, O.H., Draper, B.W., Marlow, F.L.
- ID
- ZDB-PUB-240620-35
- Date
- 2024
- Source
- Nature communications 15: 52485248 (Journal)
- Registered Authors
- Draper, Bruce, Liu, Yulong, Marlow, Florence
- Keywords
- none
- Datasets
- GEO:GSE254850
- MeSH Terms
-
- Animals
- Female
- Gene Expression Regulation, Developmental
- Male
- Mechanistic Target of Rapamycin Complex 1/genetics
- Mechanistic Target of Rapamycin Complex 1/metabolism
- Nuclear Pore Complex Proteins/genetics
- Nuclear Pore Complex Proteins/metabolism
- Nutrients/metabolism
- Oocytes*/metabolism
- Oogenesis*/genetics
- Ovary/metabolism
- RNA-Binding Proteins*/genetics
- RNA-Binding Proteins*/metabolism
- Signal Transduction
- Testis/metabolism
- Zebrafish*/genetics
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 38898112 Full text @ Nat. Commun.
Citation
Wilson, M.L., Romano, S.N., Khatri, N., Aharon, D., Liu, Y., Kaufman, O.H., Draper, B.W., Marlow, F.L. (2024) Rbpms2 promotes female fate upstream of the nutrient sensing Gator2 complex component Mios. Nature communications. 15:52485248.
Abstract
Reproductive success relies on proper establishment and maintenance of biological sex. In many animals, including mammals, the primary gonad is initially ovary biased. We previously showed the RNA binding protein (RNAbp), Rbpms2, is required for ovary fate in zebrafish. Here, we identified Rbpms2 targets in oocytes (Rbpms2-bound oocyte RNAs; rboRNAs). We identify Rbpms2 as a translational regulator of rboRNAs, which include testis factors and ribosome biogenesis factors. Further, genetic analyses indicate that Rbpms2 promotes nucleolar amplification via the mTorc1 signaling pathway, specifically through the mTorc1-activating Gap activity towards Rags 2 (Gator2) component, Missing oocyte (Mios). Cumulatively, our findings indicate that early gonocytes are in a dual poised, bipotential state in which Rbpms2 acts as a binary fate-switch. Specifically, Rbpms2 represses testis factors and promotes oocyte factors to promote oocyte progression through an essential Gator2-mediated checkpoint, thereby integrating regulation of sexual differentiation factors and nutritional availability pathways in zebrafish oogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping