PUBLICATION
Quorum-Sensing Signal DSF Inhibits the Proliferation of Intestinal Pathogenic Bacteria and Alleviates Inflammatory Response to Suppress DSS-Induced Colitis in Zebrafish
- Authors
- Yi, R., Yang, B., Zhu, H., Sun, Y., Wu, H., Wang, Z., Lu, Y., He, Y.W., Tian, J.
- ID
- ZDB-PUB-240620-20
- Date
- 2024
- Source
- Nutrients 16(11): (Journal)
- Registered Authors
- Tian, Jing
- Keywords
- diffusible signal factor (DSF), gut microbiota, inflammatory bowel disease (IBD), quorum-sensing signal, zebrafish model
- MeSH Terms
-
- Aeromonas hydrophila
- Animals
- Colitis*/chemically induced
- Colitis*/drug therapy
- Colitis*/microbiology
- Dextran Sulfate*
- Disease Models, Animal*
- Gastrointestinal Microbiome*/drug effects
- Inflammation
- Intestines/microbiology
- Quorum Sensing*/drug effects
- Staphylococcus aureus/drug effects
- Zebrafish*
- PubMed
- 38892496 Full text @ Nutrients
Citation
Yi, R., Yang, B., Zhu, H., Sun, Y., Wu, H., Wang, Z., Lu, Y., He, Y.W., Tian, J. (2024) Quorum-Sensing Signal DSF Inhibits the Proliferation of Intestinal Pathogenic Bacteria and Alleviates Inflammatory Response to Suppress DSS-Induced Colitis in Zebrafish. Nutrients. 16(11):.
Abstract
The imbalance of gut microbiota is an important factor leading to inflammatory bowel disease (IBD). Diffusible signal factor (DSF) is a novel quorum-sensing signal that regulates bacterial growth, metabolism, pathogenicity, and host immune response. This study aimed to explore the therapeutic effect and underlying mechanisms of DSF in a zebrafish colitis model induced by sodium dextran sulfate (DSS). The results showed that intake of DSF can significantly improve intestinal symptoms in the zebrafish colitis model, including ameliorating the shortening of the intestine, reducing the increase in the goblet cell number, and restoring intestinal pathological damage. DSF inhibited the upregulation of inflammation-related genes and promoted the expression of claudin1 and occludin1 to protect the tightness of intestinal tissue. The gut microbiome analysis demonstrated that DSF treatment helped the gut microbiota of the zebrafish colitis model recover to normal at the phylum and genus levels, especially in terms of pathogenic bacteria; DSF treatment downregulated the relative abundance of Aeromonas hydrophila and Staphylococcus aureus, and it was confirmed in microbiological experiments that DSF could effectively inhibit the colonization and infection of these two pathogens in the intestine. This study suggests that DSF can alleviate colitis by inhibiting the proliferation of intestinal pathogens and inflammatory responses in the intestine. Therefore, DSF has the potential to become a dietary supplement that assists in the antibiotic and nutritional treatment of IBD.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping