PUBLICATION
Identification, Expression and Evolutional Analysis of Two cyp19-like Genes in Amphioxus
- Authors
- Wang, Y., Lin, J., Li, W., Ji, G., Liu, Z.
- ID
- ZDB-PUB-240428-18
- Date
- 2024
- Source
- Animals : an open access journal from MDPI 14(8): (Journal)
- Registered Authors
- Liu, Zhenhui
- Keywords
- amphioxus, cyp19, invertebrates, sex differentiation
- MeSH Terms
- none
- PubMed
- 38672288 Full text @ Animals (Basel)
Citation
Wang, Y., Lin, J., Li, W., Ji, G., Liu, Z. (2024) Identification, Expression and Evolutional Analysis of Two cyp19-like Genes in Amphioxus. Animals : an open access journal from MDPI. 14(8):.
Abstract
The mechanism of sex determination and differentiation in animals remains a central focus of reproductive and developmental biology research, and the regulation of sex differentiation in amphioxus remains poorly understood. Cytochrome P450 Family 19 Subfamily A member 1 (CYP19A1) is a crucial sex differentiation gene that catalyzes the conversion of androgens into estrogens. In this study, we identified two aromatase-like genes in amphioxus: cyp19-like1 and cyp19-like2. The cyp19-like1 is more primitive and may represent the ancestral form of cyp19 in zebrafish and other vertebrates, while the cyp19-like2 is likely the result of gene duplication within amphioxus. To gain further insights into the expression level of these two aromatase-like, we examined their expression in different tissues and during different stages of gonad development. While the expression level of the two genes differs in tissues, both are highly expressed in the gonad primordium and are primarily localized to microsomal membrane systems. However, as development proceeds, their expression level decreases significantly. This study enhances our understanding of sex differentiation mechanisms in amphioxus and provides valuable insights into the formation and evolution of sex determination mechanisms in vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping