PUBLICATION
The structure of TRAF7 coiled-coil trimer provides insight into its function in zebrafish embryonic development
- Authors
- Song, X., Hu, R., Chen, Y., Xiao, M., Zhang, H., Wu, S., Lu, Q.
- ID
- ZDB-PUB-240105-34
- Date
- 2024
- Source
- Journal of molecular cell biology 16(1): (Journal)
- Registered Authors
- Chen, Yi
- Keywords
- TRAF7, coiled-coil domain, crystal structure, embryonic development, trimerization
- MeSH Terms
-
- Animals
- Crystallography, X-Ray
- Embryonic Development*/genetics
- Humans
- Models, Molecular
- Mutation
- Protein Multimerization
- Tumor Necrosis Factor Receptor-Associated Peptides and Proteins/genetics
- Tumor Necrosis Factor Receptor-Associated Peptides and Proteins/metabolism
- Zebrafish*/embryology
- Zebrafish*/metabolism
- Zebrafish Proteins*/chemistry
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 38178633 Full text @ J. Mol. Cell Biol.
Citation
Song, X., Hu, R., Chen, Y., Xiao, M., Zhang, H., Wu, S., Lu, Q. (2024) The structure of TRAF7 coiled-coil trimer provides insight into its function in zebrafish embryonic development. Journal of molecular cell biology. 16(1):.
Abstract
TRAF7 serves as a crucial intracellular adaptor and E3 ubiquitin ligase involved in signal transduction pathways, contributing to immune responses, tumor progression, and embryonic development. Somatic mutations within the coiled-coil (CC) domain and WD40 repeat domain of TRAF7 could cause brain tumors, while germline pathogenic mutations contribute to severe developmental abnormalities. However, the precise molecular mechanism underlying TRAF7 involvement in embryonic development remains unclear. In this study, we employed zebrafish as an in-vivo model system. TRAF7 knockdown caused defects in zebrafish embryonic development. We determined the crystal structure of TRAF7 CC domain at 3.3 Å resolution and found that the CC region trimerization was essential for TRAF7 functionality during zebrafish embryonic development. Additionally, disease-causing mutations in TRAF7 CC region could impair the trimer formation, consequently impacting early embryonic development of zebrafish. Therefore, our study sheds light on the molecular mechanism of TRAF7 CC trimer formation and its pivotal role in embryonic development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping