PUBLICATION

Alone in a crowd: effect of a nonfunctional lateral line on expression of the social hormone parathyroid hormone 2

Authors

Venuto, A., Smith, C.P., Cameron-Pack, M., Erickson, T.

ID

ZDB-PUB-220929-12

Date

2022

Source

Biology Open 11(10): (Journal)

Registered Authors

Erickson, Timothy

Keywords

Hair cells, Lateral line, Neuropeptide, Social brain, Zebrafish, pth2

MeSH Terms

Animals
Lateral Line System*/metabolism
Neuropeptides*/metabolism
Parathyroid Hormone
Receptor, Parathyroid Hormone, Type 2
Zebrafish/metabolism

(all 6)

PubMed

36161311 Full text @ Biol. Open

Abstract

Parathyroid hormone 2 (Pth2) is a vertebrate-specific neuropeptide whose thalamic expression is upregulated by social contact with conspecifics. However, social interactions fail to stimulate pth2 expression in isolated zebrafish whose lateral line hair cells have been chemically ablated. These results suggest that modulation of pth2 by social context is acutely dependent on mechanosensory information from the lateral line. However, it is unclear how a congenital loss of lateral line function influences the ability of zebrafish to interpret their social environment. In this study, we measure pth2 levels in zebrafish mutants lacking hair cell function in either the lateral line only, or in both the inner ear and lateral line. Socially-raised lateral line mutants express lower levels of pth2 relative to wild type siblings, but there is no further reduction when all sensory hair cells are nonfunctional. However, social isolation of hair cell mutants causes a further reduction in pth2 expression, pointing to additional unidentified sensory cues that influence pth2 production. Lastly, we report that social context modulates fluorescent transgenes driven by the pth2 promoter. Altogether, these data suggest that lateral line mutants experience a form of isolation, even when raised in a social environment.

Genes / Markers

Marker	Marker Type	Name
cacna1da	GENE	calcium channel, voltage-dependent, L type, alpha 1D subunit, a
lhfpl5b	GENE	LHFPL tetraspan subfamily member 5b
pcdh15a	GENE	protocadherin-related 15a
pth2	GENE	parathyroid hormone 2
tomt	GENE	transmembrane O-methyltransferase

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Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT	housing conditions	Day 4	thalamus pth2 expression decreased amount, abnormal	Fig. 3.
cacna1da^{tc323d/tc323d}	standard conditions	Day 4	thalamus pth2 expression decreased amount, abnormal	Fig. 2.
lhfpl5b^vo35/vo35	standard conditions	Day 4	thalamus pth2 expression decreased amount, abnormal	Fig. 1.
lhfpl5b^vo35/vo35	standard conditions	Day 4	thalamus pth2 expression decreased amount, abnormal	Fig. 2.
lhfpl5b^vo35/vo35	chemical treatment by environment: neomycin, chemical ablation: neuromast hair cell	Day 4	thalamus pth2 expression decreased amount, abnormal	Fig. 1.
lhfpl5b^vo35/vo35	housing conditions	Day 4	thalamus pth2 expression decreased amount, abnormal	Fig. 3.
lhfpl5b^vo35/vo35; unb2Tg	standard conditions	Day 4	thalamus EGFP expression decreased amount, abnormal	Fig. 5.
ot80Tg; unb3Tg	housing conditions	Day 4	thalamus TagRFP expression decreased amount, abnormal	Fig. 5.
pcdh15a^{th263b/th263b}	standard conditions	Day 4	thalamus pth2 expression decreased amount, abnormal	Fig. 2.
tomt^{tk256c/tk256c}	standard conditions	Day 4	thalamus pth2 expression decreased amount, abnormal	Fig. 2.

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
ot80Tg	Tg(slc6a3:EGFP)	Transgenic Insertion
tc323d		Point Mutation	cacna1da
th263b		Point Mutation	pcdh15a
tk256c		Point Mutation	tomt
unb2Tg	Tg(pth2:EGFP,cryaa:EGFP)	Transgenic Insertion
unb3Tg	Tg(pth2:TagRFP,cryaa:mCherry)	Transgenic Insertion
vo35		Small Deletion	lhfpl5b

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Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
cacna1da^{tc323d/tc323d}
lhfpl5b^vo35/vo35
lhfpl5b^vo35/vo35; unb2Tg
ot80Tg; unb3Tg
pcdh15a^{th263b/th263b}
tomt^{tk256c/tk256c}
unb2Tg
WT

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP
TagRFP	EFG	TagRFP

Mapping

Venuto et al., 2022 ZDB-PUB-220929-12 PMID:36161311

Alone in a crowd: effect of a nonfunctional lateral line on expression of the social hormone parathyroid hormone 2

Venuto et al., 2022

ZDB-PUB-220929-12
PMID:36161311