PUBLICATION

The disconnect2 mutation disrupts the tjp1b gene that is required for electrical synapse formation

Authors
Michel, J.C., Lasseigne, A.M., Marsh, A.J., Miller, A.C.
ID
ZDB-PUB-220722-2
Date
2022
Source
microPublication. Biology   2022: (Journal)
Registered Authors
Marsh, Audrey, Miller, Adam
Keywords
none
MeSH Terms
none
PubMed
35855444 Full text @ MicroPubl Biol
Abstract
To investigate electrical synapse formation in vivo we used forward genetics to disrupt genes affecting Mauthner cell electrical synapses in larval zebrafish. We identify the disconnect2 ( dis2 ) mutation for its failure to localize neural gap junction channels at electrical synapses. We mapped this mutation to chromosome 25 and identified a splice-altering mutation in the tjp1b gene. We demonstrated that the dis2 mutation disrupts tjp1b function using complementation analysis with CRISPR generated mutants. We conclude that the dis2 mutation disrupts the tjp1b gene that is required for electrical synapse formation.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping