PUBLICATION
The disconnect2 mutation disrupts the tjp1b gene that is required for electrical synapse formation
- Authors
- Michel, J.C., Lasseigne, A.M., Marsh, A.J., Miller, A.C.
- ID
- ZDB-PUB-220722-2
- Date
- 2022
- Source
- microPublication. Biology 2022: (Journal)
- Registered Authors
- Marsh, Audrey, Miller, Adam
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 35855444 Full text @ MicroPubl Biol
Citation
Michel, J.C., Lasseigne, A.M., Marsh, A.J., Miller, A.C. (2022) The disconnect2 mutation disrupts the tjp1b gene that is required for electrical synapse formation. microPublication. Biology. 2022:.
Abstract
To investigate electrical synapse formation in vivo we used forward genetics to disrupt genes affecting Mauthner cell electrical synapses in larval zebrafish. We identify the disconnect2 ( dis2 ) mutation for its failure to localize neural gap junction channels at electrical synapses. We mapped this mutation to chromosome 25 and identified a splice-altering mutation in the tjp1b gene. We demonstrated that the dis2 mutation disrupts tjp1b function using complementation analysis with CRISPR generated mutants. We conclude that the dis2 mutation disrupts the tjp1b gene that is required for electrical synapse formation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping