PUBLICATION
Polarized endosome dynamics engage cytoplasmic Par-3 that recruits dynein during asymmetric cell division
- Authors
- Zhao, X., Garcia, J.Q., Tong, K., Chen, X., Yang, B., Li, Q., Dai, Z., Shi, X., Seiple, I.B., Huang, B., Guo, S.
- ID
- ZDB-PUB-210613-1
- Date
- 2021
- Source
- Science advances 7(24): (Journal)
- Registered Authors
- Guo, Su
- Keywords
- none
- MeSH Terms
-
- Animals
- Asymmetric Cell Division*
- Caenorhabditis elegans/metabolism
- Caenorhabditis elegans Proteins*/metabolism
- Cytosol/metabolism
- Dyneins/metabolism
- Endosomes/metabolism
- Protein Serine-Threonine Kinases
- Zebrafish/metabolism
- PubMed
- 34117063 Full text @ Sci Adv
Citation
Zhao, X., Garcia, J.Q., Tong, K., Chen, X., Yang, B., Li, Q., Dai, Z., Shi, X., Seiple, I.B., Huang, B., Guo, S. (2021) Polarized endosome dynamics engage cytoplasmic Par-3 that recruits dynein during asymmetric cell division. Science advances. 7(24).
Abstract
In the developing embryos, the cortical polarity regulator Par-3 is critical for establishing Notch signaling asymmetry between daughter cells during asymmetric cell division (ACD). How cortically localized Par-3 establishes asymmetric Notch activity in the nucleus is not understood. Here, using in vivo time-lapse imaging of mitotic radial glia progenitors in the developing zebrafish forebrain, we uncover that during horizontal ACD along the anteroposterior embryonic axis, endosomes containing the Notch ligand DeltaD (Dld) move toward the cleavage plane and preferentially segregate into the posterior (subsequently basal) Notchhi daughter. This asymmetric segregation requires the activity of Par-3 and dynein motor complex. Using label retention expansion microscopy, we further detect Par-3 in the cytosol colocalizing the dynein light intermediate chain 1 (Dlic1) onto Dld endosomes. Par-3, Dlic1, and Dld are associated in protein complexes in vivo. Our data reveal an unanticipated mechanism by which cytoplasmic Par-3 directly polarizes Notch signaling components during ACD.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping