PUBLICATION
A single-cell resolution developmental atlas of hematopoietic stem and progenitor cell expansion in zebrafish
- Authors
- Xia, J., Kang, Z., Xue, Y., Ding, Y., Gao, S., Zhang, Y., Lv, P., Wang, X., Ma, D., Wang, L., Han, J.J., Liu, F.
- ID
- ZDB-PUB-210401-3
- Date
- 2021
- Source
- Proceedings of the National Academy of Sciences of the United States of America 118(14): (Journal)
- Registered Authors
- Liu, Feng, Ma, Dongyuan, Wang, Lu
- Keywords
- Gpr182, HSPC expansion, caudal hematopoietic tissue, single-cell RNA-seq, zebrafish
- Datasets
- GEO:GSE120503, GEO:GSE120578, GEO:GSE120509, GEO:GSE146404, GEO:GSE120581
- MeSH Terms
-
- Animals
- Cell Lineage
- Fetus/metabolism
- Gene Expression Profiling
- Hematopoiesis*
- Hematopoietic Stem Cells/physiology*
- Humans
- Liver/metabolism
- Mice
- Single-Cell Analysis
- Stem Cell Niche*
- Zebrafish
- PubMed
- 33785593 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Xia, J., Kang, Z., Xue, Y., Ding, Y., Gao, S., Zhang, Y., Lv, P., Wang, X., Ma, D., Wang, L., Han, J.J., Liu, F. (2021) A single-cell resolution developmental atlas of hematopoietic stem and progenitor cell expansion in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 118(14):.
Abstract
During vertebrate embryogenesis, fetal hematopoietic stem and progenitor cells (HSPCs) exhibit expansion and differentiation properties in a supportive hematopoietic niche. To profile the developmental landscape of fetal HSPCs and their local niche, here, using single-cell RNA-sequencing, we deciphered a dynamic atlas covering 28,777 cells and 9 major cell types (23 clusters) of zebrafish caudal hematopoietic tissue (CHT). We characterized four heterogeneous HSPCs with distinct lineage priming and metabolic gene signatures. Furthermore, we investigated the regulatory mechanism of CHT niche components for HSPC development, with a focus on the transcription factors and ligand-receptor networks involved in HSPC expansion. Importantly, we identified an endothelial cell-specific G protein-coupled receptor 182, followed by in vivo and in vitro functional validation of its evolutionally conserved role in supporting HSPC expansion in zebrafish and mice. Finally, comparison between zebrafish CHT and human fetal liver highlighted the conservation and divergence across evolution. These findings enhance our understanding of the regulatory mechanism underlying hematopoietic niche for HSPC expansion in vivo and provide insights into improving protocols for HSPC expansion in vitro.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping